4n2q

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==Crystal structure of THA8 in complex with Zm4 RNA==
==Crystal structure of THA8 in complex with Zm4 RNA==
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<StructureSection load='4n2q' size='340' side='right' caption='[[4n2q]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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<StructureSection load='4n2q' size='340' side='right'caption='[[4n2q]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4n2q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bradi Bradi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N2Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N2Q FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4n2q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Brachypodium_distachyon Brachypodium distachyon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N2Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N2Q FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4me2|4me2]], [[4n2s|4n2s]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BRADI2G00245, BRADI2G00270 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=15368 BRADI])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n2q OCA], [https://pdbe.org/4n2q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n2q RCSB], [https://www.ebi.ac.uk/pdbsum/4n2q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n2q ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n2q OCA], [http://pdbe.org/4n2q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n2q RCSB], [http://www.ebi.ac.uk/pdbsum/4n2q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n2q ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/I1HB13_BRADI I1HB13_BRADI]
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Thylakoid assembly 8 (THA8) is a pentatricopeptide repeat (PPR) RNA-binding protein required for the splicing of the transcript of ycf3, a gene involved in chloroplast thylakoid-membrane biogenesis. Here we report the identification of multiple THA8-binding sites in the ycf3 intron and present crystal structures of Brachypodium distachyon THA8 either free of RNA or bound to two of the identified RNA sites. The apostructure reveals a THA8 monomer with five tandem PPR repeats arranged in a planar fold. The complexes of THA8 bound to the two short RNA fragments surprisingly reveal asymmetric THA8 dimers with the bound RNAs at the dimeric interface. RNA binding induces THA8 dimerization, with a conserved G nucleotide of the bound RNAs making extensive contacts with both monomers. Together, these results establish a new model of RNA recognition by RNA-induced formation of an asymmetric dimer of a PPR protein.
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Structural basis for RNA recognition by a dimeric PPR-protein complex.,Ke J, Chen RZ, Ban T, Zhou XE, Gu X, Tan MH, Chen C, Kang Y, Brunzelle JS, Zhu JK, Melcher K, Xu HE Nat Struct Mol Biol. 2013 Nov 3. doi: 10.1038/nsmb.2710. PMID:24186060<ref>PMID:24186060</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4n2q" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bradi]]
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[[Category: Brachypodium distachyon]]
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[[Category: Ban, T]]
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[[Category: Large Structures]]
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[[Category: Brunzelle, J S]]
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[[Category: Ban T]]
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[[Category: Chen, R Z]]
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[[Category: Brunzelle JS]]
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[[Category: Gu, X]]
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[[Category: Chen RZ]]
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[[Category: Ke, J]]
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[[Category: Gu X]]
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[[Category: Melcher, K]]
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[[Category: Ke J]]
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[[Category: Xu, H E]]
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[[Category: Melcher K]]
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[[Category: Zhou, X E]]
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[[Category: Xu HE]]
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[[Category: Zhu, J K]]
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[[Category: Zhou XE]]
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[[Category: Chloroplast]]
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[[Category: Zhu JK]]
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[[Category: Group ii intron rna splicing]]
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[[Category: Small intron rna]]
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[[Category: Splicing-rna complex]]
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Current revision

Crystal structure of THA8 in complex with Zm4 RNA

PDB ID 4n2q

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