This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1ls2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:35, 14 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ls2.gif|left|200px]]
 
-
{{Structure
+
==Fitting of EF-Tu and tRNA in the Low Resolution Cryo-EM Map of an EF-Tu Ternary Complex (GDP and Kirromycin) Bound to E. coli 70S Ribosome==
-
|PDB= 1ls2 |SIZE=350|CAPTION= <scene name='initialview01'>1ls2</scene>, resolution 16.8&Aring;
+
<SX load='1ls2' size='340' side='right' viewer='molstar' caption='[[1ls2]], [[Resolution|resolution]] 16.80&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
+
<table><tr><td colspan='2'>[[1ls2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LS2 FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 16.8&#8491;</td></tr>
-
|GENE=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ls2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ls2 OCA], [https://pdbe.org/1ls2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ls2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ls2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ls2 ProSAT]</span></td></tr>
-
|DOMAIN=
+
</table>
-
|RELATEDENTRY=[[1efc|1EFC]], [[1ttt|1TTT]], [[1exm|1EXM]], [[1lu3|1lu3]]
+
== Function ==
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ls2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ls2 OCA], [http://www.ebi.ac.uk/pdbsum/1ls2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ls2 RCSB]</span>
+
[https://www.uniprot.org/uniprot/EFTU2_ECOLI EFTU2_ECOLI] This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.[HAMAP-Rule:MF_00118] May play an important regulatory role in cell growth and in the bacterial response to nutrient deprivation.[HAMAP-Rule:MF_00118]
-
}}
+
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ls/1ls2_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ls2 ConSurf].
 +
<div style="clear:both"></div>
-
'''Fitting of EF-Tu and tRNA in the Low Resolution Cryo-EM Map of an EF-Tu Ternary Complex (GDP and Kirromycin) Bound to E. coli 70S Ribosome'''
+
==See Also==
-
 
+
*[[Elongation factor 3D structures|Elongation factor 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</SX>
-
During the elongation cycle of protein biosynthesis, the specific amino acid coded for by the mRNA is delivered by a complex that is comprised of the cognate aminoacyl-tRNA, elongation factor Tu and GTP. As this ternary complex binds to the ribosome, the anticodon end of the tRNA reaches the decoding center in the 30S subunit. Here we present the cryo- electron microscopy (EM) study of an Escherichia coli 70S ribosome-bound ternary complex stalled with an antibiotic, kirromycin. In the cryo-EM map the anticodon arm of the tRNA presents a new conformation that appears to facilitate the initial codon-anticodon interaction. Furthermore, the elbow region of the tRNA is seen to contact the GTPase-associated center on the 50S subunit of the ribosome, suggesting an active role of the tRNA in the transmission of the signal prompting the GTP hydrolysis upon codon recognition.
+
-
 
+
-
==About this Structure==
+
-
1LS2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS2 OCA].
+
-
 
+
-
==Reference==
+
-
Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process., Valle M, Sengupta J, Swami NK, Grassucci RA, Burkhardt N, Nierhaus KH, Agrawal RK, Frank J, EMBO J. 2002 Jul 1;21(13):3557-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12093756 12093756]
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
 +
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Single protein]]
+
[[Category: Agrawal RK]]
-
[[Category: Agrawal, R K.]]
+
[[Category: Burkhardt N]]
-
[[Category: Burkhardt, N.]]
+
[[Category: Frank J]]
-
[[Category: Frank, J.]]
+
[[Category: Grassucci RA]]
-
[[Category: Grassucci, R A.]]
+
[[Category: Nierhaus KH]]
-
[[Category: Nierhaus, K H.]]
+
[[Category: Sengupta J]]
-
[[Category: Sengupta, J.]]
+
[[Category: Swami NK]]
-
[[Category: Swami, N K.]]
+
[[Category: Valle M]]
-
[[Category: Valle, M.]]
+
-
[[Category: 70s e coli ribosome]]
+
-
[[Category: cryo-em]]
+
-
[[Category: ef-tu]]
+
-
[[Category: ternary complex]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:06:37 2008''
+

Current revision

Fitting of EF-Tu and tRNA in the Low Resolution Cryo-EM Map of an EF-Tu Ternary Complex (GDP and Kirromycin) Bound to E. coli 70S Ribosome

1ls2, resolution 16.80Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ls2"
Personal tools