4n6w

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==X-Ray Crystal Structure of Citrate-bound PhnZ==
==X-Ray Crystal Structure of Citrate-bound PhnZ==
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<StructureSection load='4n6w' size='340' side='right' caption='[[4n6w]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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<StructureSection load='4n6w' size='340' side='right'caption='[[4n6w]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4n6w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultured_bacterium_hf130_aepn_1 Uncultured bacterium hf130_aepn_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N6W FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4n6w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium_HF130_AEPn_1 Uncultured bacterium HF130_AEPn_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N6W FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4n71|4n71]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ACU83550, ALOHA_HF130_AEPn_1_06c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=663362 uncultured bacterium HF130_AEPn_1])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6w OCA], [https://pdbe.org/4n6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n6w RCSB], [https://www.ebi.ac.uk/pdbsum/4n6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n6w ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6w OCA], [http://pdbe.org/4n6w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n6w RCSB], [http://www.ebi.ac.uk/pdbsum/4n6w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n6w ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/PHNZ_UNCHF PHNZ_UNCHF] Involved in the degradation of the organophosphonate 2-aminoethylphosphonic acid (2-AEP) (Probable). Catalyzes the cleavage of the carbon-phosphorus bond of (2-amino-1-hydroxyethyl)phosphonic acid to yield glycine and phosphate through an oxidative mechanism (PubMed:22564006, PubMed:24198335, PubMed:24706911). It reacts stereospecifically with the R-enantiomer of (2-amino-1-hydroxyethyl)phosphonic acid and is also able to use (R,R)-2-amino-1-hydroxypropylphosphonate as substrate (PubMed:24706911).<ref>PMID:22564006</ref> <ref>PMID:24198335</ref> <ref>PMID:24706911</ref> <ref>PMID:19788654</ref>
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The founding members of the HD-domain protein superfamily are phosphohydrolases, and newly discovered members are generally annotated as such. However, myo-inositol oxygenase (MIOX) exemplifies a second, very different function that has evolved within the common scaffold of this superfamily. A recently discovered HD protein, PhnZ, catalyzes conversion of 2-amino-1-hydroxyethylphosphonate to glycine and phosphate, culminating a bacterial pathway for the utilization of environmentally abundant 2-aminoethylphosphonate. Using Mossbauer and EPR spectroscopies, X-ray crystallography, and activity measurements, we show here that, like MIOX, PhnZ employs a mixed-valent Fe(II)/Fe(III) cofactor for the O2-dependent oxidative cleavage of its substrate. Phylogenetic analysis suggests that many more HD proteins may catalyze yet-unknown oxygenation reactions using this hitherto exceptional Fe(II)/Fe(III) cofactor. The results demonstrate that the catalytic repertoire of the HD superfamily extends well beyond phosphohydrolysis and suggest that the mechanism used by MIOX and PhnZ may be a common strategy for oxidative C-X bond cleavage.
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Organophosphonate-degrading PhnZ reveals an emerging family of HD domain mixed-valent diiron oxygenases.,Worsdorfer B, Lingaraju M, Yennawar NH, Boal AK, Krebs C, Bollinger JM Jr, Pandelia ME Proc Natl Acad Sci U S A. 2013 Nov 19;110(47):18874-9. doi:, 10.1073/pnas.1315927110. Epub 2013 Nov 6. PMID:24198335<ref>PMID:24198335</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4n6w" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Uncultured bacterium hf130_aepn_1]]
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[[Category: Large Structures]]
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[[Category: Boal, A K]]
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[[Category: Uncultured bacterium HF130_AEPn_1]]
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[[Category: Jr, J M.Bollinger]]
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[[Category: Boal AK]]
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[[Category: Krebs, C]]
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[[Category: Bollinger Jr JM]]
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[[Category: Lingaraju, M]]
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[[Category: Krebs C]]
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[[Category: Pandelia, M E]]
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[[Category: Lingaraju M]]
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[[Category: Worsdorfer, B]]
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[[Category: Pandelia ME]]
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[[Category: Yennawar, N H]]
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[[Category: Worsdorfer B]]
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[[Category: Oxidoreductase]]
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[[Category: Yennawar NH]]
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[[Category: Oxygenase]]
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Current revision

X-Ray Crystal Structure of Citrate-bound PhnZ

PDB ID 4n6w

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