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| | ==The structure of apo bradavidin2 (Form A)== | | ==The structure of apo bradavidin2 (Form A)== |
| - | <StructureSection load='4ggr' size='340' side='right' caption='[[4ggr]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='4ggr' size='340' side='right'caption='[[4ggr]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ggr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bradu Bradu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GGR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GGR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ggr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_diazoefficiens_USDA_110 Bradyrhizobium diazoefficiens USDA 110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GGR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GGR FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2y32|2y32]], [[4ggt|4ggt]], [[4ggz|4ggz]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bll1558 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224911 BRADU])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ggr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ggr OCA], [https://pdbe.org/4ggr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ggr RCSB], [https://www.ebi.ac.uk/pdbsum/4ggr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ggr ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ggr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ggr OCA], [http://pdbe.org/4ggr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ggr RCSB], [http://www.ebi.ac.uk/pdbsum/4ggr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ggr ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q89U61_BRADU Q89U61_BRADU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Avidin|Avidin]] | + | *[[Avidin 3D structures|Avidin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bradu]] | + | [[Category: Bradyrhizobium diazoefficiens USDA 110]] |
| - | [[Category: Livnah, O]] | + | [[Category: Large Structures]] |
| - | [[Category: Meir, A]] | + | [[Category: Livnah O]] |
| - | [[Category: Avidin]] | + | [[Category: Meir A]] |
| - | [[Category: Beta barrel]]
| + | |
| - | [[Category: Biotin binding protein]]
| + | |
| - | [[Category: Bradavidin]]
| + | |
| - | [[Category: High affinity system]]
| + | |
| - | [[Category: Lipocalin fold]]
| + | |
| - | [[Category: Oligomeric state]]
| + | |
| - | [[Category: Streptavidin]]
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| Structural highlights
Function
Q89U61_BRADU
Publication Abstract from PubMed
Bradavidin II is a biotin-binding protein from Bradyrhizobium japonicum that resembles chicken avidin and bacterial streptavidin. A biophysical characterization was carried out using dynamic light scattering, native mass spectrometry, differential scanning calorimetry, and isothermal titration calorimetry combined with structural characterization using X-ray crystallography. These observations revealed that bradavidin II differs from canonical homotetrameric avidin protein family members in its quaternary structure. In contrast with the other avidins, bradavidin II appears to have a dynamic (transient) oligomeric state in solution. It is monomeric at low protein concentrations but forms higher oligomeric assemblies at higher concentrations. The crystal structure of bradavidin II revealed an important role for Phe42 in shielding the bound ligand from surrounding water molecules, thus functionally replacing the L7,8 loop essential for tight ligand binding in avidin and streptavidin. This bradavidin II characterization opens new avenues for oligomerization-independent biotin-binding protein development.
The highly dynamic oligomeric structure of bradavidin II is unique among avidin proteins.,Leppiniemi J, Meir A, Kahkonen N, Kukkurainen S, Maatta JA, Ojanen M, Janis J, S Kulomaa M, Livnah O, Hytonen VP Protein Sci. 2013 May 10. doi: 10.1002/pro.2281. PMID:23661323[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Leppiniemi J, Meir A, Kahkonen N, Kukkurainen S, Maatta JA, Ojanen M, Janis J, S Kulomaa M, Livnah O, Hytonen VP. The highly dynamic oligomeric structure of bradavidin II is unique among avidin proteins. Protein Sci. 2013 May 10. doi: 10.1002/pro.2281. PMID:23661323 doi:10.1002/pro.2281
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