|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Structural Analysis of Adhesive Tip pilin, GBS104 from Group B Streptococcus agalactiae== | | ==Structural Analysis of Adhesive Tip pilin, GBS104 from Group B Streptococcus agalactiae== |
| - | <StructureSection load='3tvy' size='340' side='right' caption='[[3tvy]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='3tvy' size='340' side='right'caption='[[3tvy]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3tvy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_(serotype_v) Streptococcus agalactiae (serotype v)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TVY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TVY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3tvy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_V Streptococcus agalactiae serogroup V]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TVY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TVY FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3tw0|3tw0]], [[3txa|3txa]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAG0649 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216466 Streptococcus agalactiae (serotype V)])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tvy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tvy OCA], [https://pdbe.org/3tvy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tvy RCSB], [https://www.ebi.ac.uk/pdbsum/3tvy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tvy ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tvy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tvy OCA], [http://pdbe.org/3tvy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tvy RCSB], [http://www.ebi.ac.uk/pdbsum/3tvy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tvy ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q8E0S5_STRA5 Q8E0S5_STRA5] |
| - | The crystal structure of a 75 kDa central fragment of GBS104, a tip pilin from the 2063V/R strain of Streptococcus agalactiae (group B streptococcus; GBS), is reported. In addition, a homology model of the remaining two domains of GBS104 was built and a model of full-length GBS104 was generated by combining the homology model (the N1 and N4 domains) and the crystal structure of the 75 kDa fragment (the N2 and N3 domains). This rod-shaped GBS104 model is constructed of three IgG-like domains (the N1, N2 and N4 domains) and one vWFA-like domain (the N3 domain). The N1 and N2 domains of GBS104 are assembled with distinct and remote segments contributed by the N- and C-termini. The metal-binding site in the N3 domain of GBS104 is in the closed/low-affinity conformation. Interestingly, this domain hosts two long arms that project away from the metal-binding site. Using site-directed mutagenesis, two cysteine residues that lock the N3 domain of GBS104 into the open/high-affinity conformation were introduced. Both wild-type and disulfide-locked recombinant proteins were tested for binding to extracellular matrix proteins such as collagen, fibronectin, fibrinogen and laminin, and an increase in fibronectin binding affinity was identified for the disulfide-locked N3 domain, suggesting that induced conformational changes may play a possible role in receptor binding.
| + | |
| | | | |
| - | Structure of Streptococcus agalactiae tip pilin GBS104: a model for GBS pili assembly and host interactions.,Krishnan V, Dwivedi P, Kim BJ, Samal A, Macon K, Ma X, Mishra A, Doran KS, Ton-That H, Narayana SV Acta Crystallogr D Biol Crystallogr. 2013 Jun;69(Pt 6):1073-89. doi:, 10.1107/S0907444913004642. Epub 2013 May 15. PMID:23695252<ref>PMID:23695252</ref>
| + | ==See Also== |
| - | | + | *[[Pilin 3D structures|Pilin 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3tvy" style="background-color:#fffaf0;"></div>
| + | |
| - | == References == | + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Krishnan, V]] | + | [[Category: Large Structures]] |
| - | [[Category: Narayana, S V.L]] | + | [[Category: Streptococcus agalactiae serogroup V]] |
| - | [[Category: Ancillary pilin]] | + | [[Category: Krishnan V]] |
| - | [[Category: Cell adhesion]] | + | [[Category: Narayana SVL]] |
| - | [[Category: Gram-positive bacterial cell surface]]
| + | |
| - | [[Category: Vwfa fold]]
| + | |
