3h4r

<StructureSection load='3h4r' size='340' side='right' caption='[[3h4r]], [[Resolution|resolution]] 2.80&Aring;' scene=''>

<table><tr><td colspan='2'>[[3h4r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3H4R FirstGlance]. <br>

</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b1350, JW1344, recE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3h4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h4r OCA], [http://pdbe.org/3h4r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3h4r RCSB], [http://www.ebi.ac.uk/pdbsum/3h4r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3h4r ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/RECE_ECOLI RECE_ECOLI]] Is involved in the RecE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA.

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h4/3h4r_consurf.spt"</scriptWhenChecked>

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== Publication Abstract from PubMed ==

Escherichia coli RecE protein is part of the classical RecET recombination system that has recently been used in powerful new methods for genetic engineering. RecE binds to free double-stranded DNA (dsDNA) ends and processively digests the 5'-ended strand to form 5'-mononucleotides and a 3'-overhang that is a substrate for single strand annealing promoted by RecT. Here, we report the crystal structure of the C-terminal nuclease domain of RecE at 2.8 A resolution. RecE forms a toroidal tetramer with a central tapered channel that is wide enough to bind dsDNA at one end, but is partially plugged at the other end by the C-terminal segment of the protein. Four narrow tunnels, one within each subunit of the tetramer, lead from the central channel to the four active sites, which lie about 15 A from the channel. The structure, combined with mutational studies, suggests a mechanism in which dsDNA enters through the open end of the central channel, the 5'-ended strand passes through a tunnel to access one of the four active sites, and the 3'-ended strand passes through the plugged end of the channel at the back of the tetramer.

Crystal structure of E. coli RecE protein reveals a toroidal tetramer for processing double-stranded DNA breaks.,Zhang J, Xing X, Herr AB, Bell CE Structure. 2009 May 13;17(5):690-702. PMID:19446525<ref>PMID:19446525</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 3h4r" style="background-color:#fffaf0;"></div>

*[[Exonuclease|Exonuclease]]

[[Category: Ecoli]]

[[Category: Bell, C E]]

[[Category: Zhang, J]]

[[Category: Exonuclease]]

[[Category: Recombination]]