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| | ==Structure analysis of Geranyl diphosphate methyltransferase in complex with SAM== | | ==Structure analysis of Geranyl diphosphate methyltransferase in complex with SAM== |
| - | <StructureSection load='4f84' size='340' side='right' caption='[[4f84]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='4f84' size='340' side='right'caption='[[4f84]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4f84]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/'streptomyces_lasaliensis' 'streptomyces lasaliensis']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F84 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F84 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4f84]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_lasalocidi Streptomyces lasalocidi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F84 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F84 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4f85|4f85]], [[4f86|4f86]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gdpmt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=324833 'Streptomyces lasaliensis'])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f84 OCA], [https://pdbe.org/4f84 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f84 RCSB], [https://www.ebi.ac.uk/pdbsum/4f84 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f84 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f84 OCA], [http://pdbe.org/4f84 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f84 RCSB], [http://www.ebi.ac.uk/pdbsum/4f84 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f84 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GPPMT_STRLS GPPMT_STRLS]] Catalyzes the SAM-dependent methylation of geranyl diphosphate (GPP) to yield (E)-2-methylgeranyl diphosphate (2-MeGPP). | + | [https://www.uniprot.org/uniprot/GPPMT_STRLS GPPMT_STRLS] Catalyzes the SAM-dependent methylation of geranyl diphosphate (GPP) to yield (E)-2-methylgeranyl diphosphate (2-MeGPP). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Streptomyces lasaliensis]] | + | [[Category: Large Structures]] |
| - | [[Category: Ariyawutthiphan, O]] | + | [[Category: Streptomyces lasalocidi]] |
| - | [[Category: Gao, Y G]] | + | [[Category: Ariyawutthiphan O]] |
| - | [[Category: Minami, A]] | + | [[Category: Gao YG]] |
| - | [[Category: Oikawa, H]] | + | [[Category: Minami A]] |
| - | [[Category: Ose, T]] | + | [[Category: Oikawa H]] |
| - | [[Category: Tanaka, I]] | + | [[Category: Ose T]] |
| - | [[Category: Yao, M]] | + | [[Category: Tanaka I]] |
| - | [[Category: Alpha/beta]]
| + | [[Category: Yao M]] |
| - | [[Category: Methyltransferase]]
| + | |
| - | [[Category: Rossmann fold]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
GPPMT_STRLS Catalyzes the SAM-dependent methylation of geranyl diphosphate (GPP) to yield (E)-2-methylgeranyl diphosphate (2-MeGPP).
Publication Abstract from PubMed
In the typical isoprenoid-biosynthesis pathway, condensation of the universal C(5)-unit precursors isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) occurs via the common intermediates prenyl pyrophosphates (C(10)-C(20)). The diversity of isoprenoids reflects differences in chain length, cyclization and further additional modification after cyclization. In contrast, the biosynthesis of 2-methylisonorneol (2-MIB), which is responsible for taste and odour problems in drinking water, is unique in that it primes the enzymatic methylation of geranyl pyrophosphate (GPP) before cyclization, which is catalyzed by an S-adenosyl-L-methionine-dependent methyltransferase (GPPMT). The substrate of GPPMT contains a nonconjugated olefin and the reaction mechanism is expected to be similar to that of the steroid methyltransferase (SMT) family. Here, structural analysis of GPPMT in complex with its cofactor and substrate revealed the mechanisms of substrate recognition and possible enzymatic reaction. Using the structures of these complexes, methyl-group transfer and the subsequent proton-abstraction mechanism are discussed. GPPMT and SMTs contain a conserved glutamate residue that is likely to play a role as a general base. Comparison with the reaction mechanism of the mycolic acid cyclopropane synthase (MACS) family also supports this result. This enzyme represented here is the first model of the enzymatic C-methylation of a nonconjugated olefin in the isoprenoid-biosynthesis pathway. In addition, an elaborate system to avoid methylation of incorrect substrates is proposed.
Structure analysis of geranyl pyrophosphate methyltransferase and the proposed reaction mechanism of SAM-dependent C-methylation.,Ariyawutthiphan O, Ose T, Minami A, Sinde S, Tsuda M, Gao YG, Yao M, Oikawa H, Tanaka I Acta Crystallogr D Biol Crystallogr. 2012 Nov;68(Pt 11):1558-69. doi:, 10.1107/S0907444912038486. Epub 2012 Oct 18. PMID:23090405[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ariyawutthiphan O, Ose T, Minami A, Sinde S, Tsuda M, Gao YG, Yao M, Oikawa H, Tanaka I. Structure analysis of geranyl pyrophosphate methyltransferase and the proposed reaction mechanism of SAM-dependent C-methylation. Acta Crystallogr D Biol Crystallogr. 2012 Nov;68(Pt 11):1558-69. doi:, 10.1107/S0907444912038486. Epub 2012 Oct 18. PMID:23090405 doi:10.1107/S0907444912038486
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