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| | ==Crystal Structure of HIV gp41 with MPER== | | ==Crystal Structure of HIV gp41 with MPER== |
| - | <StructureSection load='3k9a' size='340' side='right' caption='[[3k9a]], [[Resolution|resolution]] 2.10Å' scene=''> | + | <StructureSection load='3k9a' size='340' side='right'caption='[[3k9a]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3k9a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K9A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K9A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3k9a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K9A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K9A FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gp41 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k9a OCA], [http://pdbe.org/3k9a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3k9a RCSB], [http://www.ebi.ac.uk/pdbsum/3k9a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3k9a ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k9a OCA], [https://pdbe.org/3k9a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k9a RCSB], [https://www.ebi.ac.uk/pdbsum/3k9a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k9a ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
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| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k9/3k9a_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k9/3k9a_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
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| | ==See Also== | | ==See Also== |
| - | *[[Gp41|Gp41]] | + | *[[Gp41 3D Structures|Gp41 3D Structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chance, M R]] | + | [[Category: Human immunodeficiency virus 1]] |
| - | [[Category: Cho, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Habte, H]] | + | [[Category: Chance MR]] |
| - | [[Category: Han, D]] | + | [[Category: Cho M]] |
| - | [[Category: Shi, W]] | + | [[Category: Habte H]] |
| - | [[Category: Gp41]] | + | [[Category: Han D]] |
| - | [[Category: Hiv]] | + | [[Category: Shi W]] |
| - | [[Category: Membrane proximal external region]]
| + | |
| - | [[Category: Mper]]
| + | |
| - | [[Category: Viral protein]]
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| Structural highlights
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Human immunodeficiency virus, type 1 (HIV-1) envelope glycoprotein (gp120/gp41) plays a critical role in virus infection and pathogenesis. Three of the six monoclonal antibodies considered to have broadly neutralizing activities (2F5, 4E10, and Z13e1) bind to the membrane-proximal external region (MPER) of gp41. This makes the MPER a desirable template for developing immunogens that can elicit antibodies with properties similar to these monoclonal antibodies, with a long term goal of developing antigens that could serve as novel HIV vaccines. In order to provide a structural basis for rational antigen design, an MPER construct, HR1-54Q, was generated for x-ray crystallographic and x-ray footprinting studies to provide both high resolution atomic coordinates and verification of the solution state of the antigen, respectively. The crystal structure of HR1-54Q reveals a trimeric, coiled-coil six-helical bundle, which probably represents a postfusion form of gp41. The MPER portion extends from HR2 in continuation of a slightly bent long helix and is relatively flexible. The structures observed for the 2F5 and 4E10 epitopes agree well with existing structural data, and enzyme-linked immunosorbent assays indicate that the antigen binds well to antibodies that recognize the above epitopes. Hydroxyl radical-mediated protein footprinting of the antigen in solution reveals specifically protected and accessible regions consistent with the predictions based on the trimeric structure from the crystallographic data. Overall, the HR1-54Q antigen, as characterized by crystallography and footprinting, represents a postfusion, trimeric form of HIV gp41, and its structure provides a rational basis for gp41 antigen design suitable for HIV vaccine development.
Structural characterization of HIV gp41 with the membrane-proximal external region.,Shi W, Bohon J, Han DP, Habte H, Qin Y, Cho MW, Chance MR J Biol Chem. 2010 Jul 30;285(31):24290-8. Epub 2010 Jun 4. PMID:20525690[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Shi W, Bohon J, Han DP, Habte H, Qin Y, Cho MW, Chance MR. Structural characterization of HIV gp41 with the membrane-proximal external region. J Biol Chem. 2010 Jul 30;285(31):24290-8. Epub 2010 Jun 4. PMID:20525690 doi:10.1074/jbc.M110.111351
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