1lv2

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[[Image:1lv2.gif|left|200px]]
 
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{{Structure
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==Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids==
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|PDB= 1lv2 |SIZE=350|CAPTION= <scene name='initialview01'>1lv2</scene>, resolution 2.7&Aring;
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<StructureSection load='1lv2' size='340' side='right'caption='[[1lv2]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>
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<table><tr><td colspan='2'>[[1lv2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LV2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LV2 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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|GENE= HNF4G ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lv2 OCA], [https://pdbe.org/1lv2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lv2 RCSB], [https://www.ebi.ac.uk/pdbsum/1lv2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lv2 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lv2 OCA], [http://www.ebi.ac.uk/pdbsum/1lv2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lv2 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/HNF4G_HUMAN HNF4G_HUMAN] Transcription factor. Has a lower transcription activation potential than HNF4-alpha.
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== Evolutionary Conservation ==
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'''Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lv/1lv2_consurf.spt"</scriptWhenChecked>
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The 2.7 A X-ray crystal structure of the HNF4gamma ligand binding domain (LBD) revealed the presence of a fatty acid within the pocket, with the AF2 helix in a conformation characteristic of a transcriptionally active nuclear receptor. GC/MS and NMR analysis of chloroform/methanol extracts from purified HNF4alpha and HNF4gamma LBDs identified mixtures of saturated and cis-monounsaturated C14-18 fatty acids. The purified HNF4 LBDs interacted with nuclear receptor coactivators, and both HNF4 subtypes show high constitutive activity in transient transfection assays, which was reduced by mutations designed to interfere with fatty acid binding. The endogenous fatty acids did not readily exchange with radiolabeled palmitic acid, and all attempts to displace them without denaturing the protein failed. Our results suggest that the HNF4s may be transcription factors that are constitutively bound to fatty acids.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1LV2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LV2 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lv2 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Hepatocyte nuclear factor 4 is a transcription factor that constitutively binds fatty acids., Wisely GB, Miller AB, Davis RG, Thornquest AD Jr, Johnson R, Spitzer T, Sefler A, Shearer B, Moore JT, Miller AB, Willson TM, Williams SP, Structure. 2002 Sep;10(9):1225-34. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12220494 12220494]
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Davis, R G.]]
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[[Category: Davis RG]]
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[[Category: Johnson, R.]]
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[[Category: Johnson R]]
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[[Category: Miller, A B.]]
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[[Category: Miller AB]]
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[[Category: Moore, J T.]]
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[[Category: Moore JT]]
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[[Category: Sefler, A.]]
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[[Category: Sefler A]]
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[[Category: Shearer, B.]]
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[[Category: Shearer B]]
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[[Category: Spitzer, T.]]
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[[Category: Spitzer T]]
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[[Category: Williams, S P.]]
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[[Category: Williams SP]]
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[[Category: Willson, T M.]]
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[[Category: Willson TM]]
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[[Category: Wisely, B.]]
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[[Category: Wisely B]]
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[[Category: diabetes]]
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[[Category: fatty acid]]
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[[Category: hnf4]]
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[[Category: mody]]
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[[Category: nuclear receptor]]
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[[Category: transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:07:44 2008''
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Current revision

Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids

PDB ID 1lv2

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