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3ong

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==Crystal structure of UBA2ufd-Ubc9: insights into E1-E2 interactions in Sumo pathways==
==Crystal structure of UBA2ufd-Ubc9: insights into E1-E2 interactions in Sumo pathways==
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<StructureSection load='3ong' size='340' side='right' caption='[[3ong]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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<StructureSection load='3ong' size='340' side='right'caption='[[3ong]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3ong]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ONG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ONG FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3ong]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ONG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ONG FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3onh|3onh]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UBA2, PIP2, UAL1, YDR390C, D9509.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), UBC9, YDL064W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ong FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ong OCA], [https://pdbe.org/3ong PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ong RCSB], [https://www.ebi.ac.uk/pdbsum/3ong PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ong ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ong FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ong OCA], [http://pdbe.org/3ong PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ong RCSB], [http://www.ebi.ac.uk/pdbsum/3ong PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ong ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/UBA2_YEAST UBA2_YEAST]] The dimeric enzyme acts as a SMT3 E1 ligase. It mediates ATP-dependent activation of SMT3 and formation of a thioester with a conserved cysteine residue on AOS1. [[http://www.uniprot.org/uniprot/UBC9_YEAST UBC9_YEAST]] E2 ubiquitin-like--protein ligase mediating SUMO/Smt3 attachment to septins and PCNA. Seems to be involved in degradation of S- (CLB5) and M-phase cyclins (CLB2).<ref>PMID:9341106</ref> <ref>PMID:11572779</ref>
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[https://www.uniprot.org/uniprot/UBA2_YEAST UBA2_YEAST] The dimeric enzyme acts as a SMT3 E1 ligase. It mediates ATP-dependent activation of SMT3 and formation of a thioester with a conserved cysteine residue on AOS1.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Canonical ubiquitin-like proteins (UBLs) such as ubiquitin, Sumo, NEDD8, and ISG15 are ligated to targets by E1-E2-E3 multienzyme cascades. The Sumo cascade, conserved among all eukaryotes, regulates numerous biological processes including protein localization, transcription, DNA replication, and mitosis. Sumo conjugation is initiated by the heterodimeric Aos1-Uba2 E1 enzyme (in humans called Sae1-Uba2), which activates Sumo's C-terminus, binds the dedicated E2 enzyme Ubc9, and promotes Sumo C-terminal transfer between the Uba2 and Ubc9 catalytic cysteines. To gain insights into details of E1-E2 interactions in the Sumo pathway, we determined crystal structures of the C-terminal ubiquitin fold domain (ufd) from yeast Uba2 (Uba2(ufd)), alone and in complex with Ubc9. The overall structures of both yeast Uba2(ufd) and Ubc9 superimpose well on their individual human counterparts, suggesting conservation of fundamental features of Sumo conjugation. Docking the Uba2(ufd)-Ubc9 and prior full-length human Uba2 structures allows generation of models for steps in Sumo transfer from Uba2 to Ubc9, and supports the notion that Uba2 undergoes remarkable conformational changes during the reaction. Comparisons to previous structures from the NEDD8 cascade demonstrate that UBL cascades generally utilize some parallel E1-E2 interaction surfaces. In addition, the structure of the Uba2(ufd)-Ubc9 complex reveals interactions unique to Sumo E1 and E2. Comparison with a previous Ubc9-E3 complex structure demonstrates overlap between Uba2 and E3 binding sites on Ubc9, indicating that loading with Sumo and E3-catalyzed transfer to substrates are strictly separate steps. The results suggest mechanisms establishing specificity and order in Sumo conjugation cascades.
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Crystal structure of UBA2(ufd)-Ubc9: insights into E1-E2 interactions in Sumo pathways.,Wang J, Taherbhoy AM, Hunt HW, Seyedin SN, Miller DW, Miller DJ, Huang DT, Schulman BA PLoS One. 2010 Dec 30;5(12):e15805. PMID:21209884<ref>PMID:21209884</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ong" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[SUMO conjugating enzyme Ubc9|SUMO conjugating enzyme Ubc9]]
*[[SUMO conjugating enzyme Ubc9|SUMO conjugating enzyme Ubc9]]
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*[[Ubiquitin activating enzyme|Ubiquitin activating enzyme]]
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*[[3D structures of Ubiquitin activating enzyme|3D structures of Ubiquitin activating enzyme]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Atcc 18824]]
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[[Category: Large Structures]]
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[[Category: Huang, D T]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Hunt, H W]]
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[[Category: Huang DT]]
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[[Category: Miller, D W]]
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[[Category: Hunt HW]]
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[[Category: Schulman, B A]]
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[[Category: Miller DW]]
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[[Category: Seyedin, S N]]
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[[Category: Schulman BA]]
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[[Category: Taherbhoy, A M]]
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[[Category: Seyedin SN]]
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[[Category: Wang, J]]
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[[Category: Taherbhoy AM]]
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[[Category: Ligase]]
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[[Category: Wang J]]

Current revision

Crystal structure of UBA2ufd-Ubc9: insights into E1-E2 interactions in Sumo pathways

PDB ID 3ong

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