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| | ==Crystal Structure of human gamma-tubulin complex protein 4 (GCP4)== | | ==Crystal Structure of human gamma-tubulin complex protein 4 (GCP4)== |
| - | <StructureSection load='3rip' size='340' side='right' caption='[[3rip]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='3rip' size='340' side='right'caption='[[3rip]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3rip]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RIP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3rip]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RIP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TUBGCP4, 76P, GCP4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rip OCA], [http://pdbe.org/3rip PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rip RCSB], [http://www.ebi.ac.uk/pdbsum/3rip PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rip ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rip OCA], [https://pdbe.org/3rip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rip RCSB], [https://www.ebi.ac.uk/pdbsum/3rip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rip ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GCP4_HUMAN GCP4_HUMAN]] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. | + | [https://www.uniprot.org/uniprot/GCP4_HUMAN GCP4_HUMAN] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Tubulin|Tubulin]] | + | *[[Tubulin 3D Structures|Tubulin 3D Structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Gregory-Pauron, L]] | + | [[Category: Large Structures]] |
| - | [[Category: Guillet, V]] | + | [[Category: Gregory-Pauron L]] |
| - | [[Category: Mourey, L]] | + | [[Category: Guillet V]] |
| - | [[Category: Gamma-tubulin ring complex]]
| + | [[Category: Mourey L]] |
| - | [[Category: Gamma-turc]]
| + | |
| - | [[Category: Helix bundle]]
| + | |
| - | [[Category: Structural protein]]
| + | |
| Structural highlights
Function
GCP4_HUMAN Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.
Publication Abstract from PubMed
Microtubule nucleation in all eukaryotes involves gamma-tubulin small complexes (gammaTuSCs) that comprise two molecules of gamma-tubulin bound to gamma-tubulin complex proteins (GCPs) GCP2 and GCP3. In many eukaryotes, multiple gammaTuSCs associate with GCP4, GCP5 and GCP6 into large gamma-tubulin ring complexes (gammaTuRCs). Recent cryo-EM studies indicate that a scaffold similar to gammaTuRCs is formed by lateral association of gammaTuSCs, with the C-terminal regions of GCP2 and GCP3 binding gamma-tubulin molecules. However, the exact role of GCPs in microtubule nucleation remains unknown. Here we report the crystal structure of human GCP4 and show that its C-terminal domain binds directly to gamma-tubulin. The human GCP4 structure is the prototype for all GCPs, as it can be precisely positioned within the gammaTuSC envelope, revealing the nature of protein-protein interactions and conformational changes regulating nucleation activity.
Crystal structure of gamma-tubulin complex protein GCP4 provides insight into microtubule nucleation.,Guillet V, Knibiehler M, Gregory-Pauron L, Remy MH, Chemin C, Raynaud-Messina B, Bon C, Kollman JM, Agard DA, Merdes A, Mourey L Nat Struct Mol Biol. 2011 Jul 3;18(8):915-9. doi: 10.1038/nsmb.2083. PMID:21725292[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Guillet V, Knibiehler M, Gregory-Pauron L, Remy MH, Chemin C, Raynaud-Messina B, Bon C, Kollman JM, Agard DA, Merdes A, Mourey L. Crystal structure of gamma-tubulin complex protein GCP4 provides insight into microtubule nucleation. Nat Struct Mol Biol. 2011 Jul 3;18(8):915-9. doi: 10.1038/nsmb.2083. PMID:21725292 doi:10.1038/nsmb.2083
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