1m0k

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[[Image:1m0k.gif|left|200px]]
 
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{{Structure
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==BACTERIORHODOPSIN K INTERMEDIATE AT 1.43 A RESOLUTION==
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|PDB= 1m0k |SIZE=350|CAPTION= <scene name='initialview01'>1m0k</scene>, resolution 1.43&Aring;
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<StructureSection load='1m0k' size='340' side='right'caption='[[1m0k]], [[Resolution|resolution]] 1.43&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=LI1:1-[2,6,10.14-TETRAMETHYL-HEXADECAN-16-YL]-2-[2,10,14-TRIMETHYLHEXADECAN-16-YL]GLYCEROL'>LI1</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene>, <scene name='pdbligand=SQU:2,10,23-TRIMETHYL-TETRACOSANE'>SQU</scene>
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<table><tr><td colspan='2'>[[1m0k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M0K FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.43&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LI1:1-[2,6,10.14-TETRAMETHYL-HEXADECAN-16-YL]-2-[2,10,14-TRIMETHYLHEXADECAN-16-YL]GLYCEROL'>LI1</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene>, <scene name='pdbligand=SQU:2,10,23-TRIMETHYL-TETRACOSANE'>SQU</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m0k OCA], [https://pdbe.org/1m0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m0k RCSB], [https://www.ebi.ac.uk/pdbsum/1m0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m0k ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1m0l|1M0L]], [[1m0m|1M0M]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m0k OCA], [http://www.ebi.ac.uk/pdbsum/1m0k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1m0k RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA] Light-driven proton pump.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m0/1m0k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m0k ConSurf].
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<div style="clear:both"></div>
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'''BACTERIORHODOPSIN K INTERMEDIATE AT 1.43 A RESOLUTION'''
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==See Also==
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*[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The K state, an early intermediate of the bacteriorhodopsin photocycle, contains the excess free energy used for light-driven proton transport. The energy gain must reside in or near the photoisomerized retinal, but in what form has long been an open question. We produced the K intermediate in bacteriorhodopsin crystals in a photostationary state at 100K, with 40% yield, and determined its X-ray diffraction structure to 1.43 A resolution. In independent refinements of data from four crystals, the changes are confined mainly to the photoisomerized retinal. The retinal is 13-cis,15-anti, as known from vibrational spectroscopy. The C13=C14 bond is rotated nearly fully to cis from the initial trans configuration, but the C14-C15 and C15=NZ bonds are partially counter-rotated. This strained geometry keeps the direction of the Schiff base N-H bond vector roughly in the extracellular direction, but the angle of its hydrogen bond with water 402, that connects it to the anionic Asp85 and Asp212, is not optimal. Weakening of this hydrogen bond may account for many of the reported features of the infrared spectrum of K, and for its photoelectric signal, as well as the deprotonation of the Schiff base later in the cycle. Importantly, although 13-cis, the retinal does not assume the expected bent shape of this configuration. Comparison of the calculated energy of the increased angle of C12-C13=C14, that allows this distortion, with the earlier reported calorimetric measurement of the enthalpy gain of the K state indicates that a significant part of the excess energy is conserved in the bond strain at C13.
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==About this Structure==
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1M0K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M0K OCA].
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==Reference==
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Crystallographic structure of the K intermediate of bacteriorhodopsin: conservation of free energy after photoisomerization of the retinal., Schobert B, Cupp-Vickery J, Hornak V, Smith S, Lanyi J, J Mol Biol. 2002 Aug 23;321(4):715-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12206785 12206785]
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[[Category: Halobacterium salinarum]]
[[Category: Halobacterium salinarum]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Lanyi, J K.]]
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[[Category: Lanyi JK]]
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[[Category: 7-transmembrane]]
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[[Category: haloarchaea]]
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[[Category: ion pump]]
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[[Category: lipid]]
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[[Category: membrane protein]]
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[[Category: merohedral twinning]]
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[[Category: photoreceptor]]
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[[Category: retinal protein]]
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[[Category: serpentine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:09:40 2008''
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Current revision

BACTERIORHODOPSIN K INTERMEDIATE AT 1.43 A RESOLUTION

PDB ID 1m0k

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