1m0g
From Proteopedia
(Difference between revisions)
| (10 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | [[Image:1m0g.jpg|left|200px]] | ||
| - | + | ==Solution structure of the alpha domain of mt_nc== | |
| - | + | <StructureSection load='1m0g' size='340' side='right'caption='[[1m0g]]' scene=''> | |
| - | | | + | == Structural highlights == |
| - | | | + | <table><tr><td colspan='2'>[[1m0g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Notothenia_coriiceps Notothenia coriiceps]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M0G FirstGlance]. <br> |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m0g OCA], [https://pdbe.org/1m0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m0g RCSB], [https://www.ebi.ac.uk/pdbsum/1m0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m0g ProSAT]</span></td></tr> | |
| - | + | </table> | |
| - | + | == Function == | |
| - | + | [https://www.uniprot.org/uniprot/MTA_NOTNE MTA_NOTNE] Metallothioneins have a high content of cysteine residues that bind various heavy metals. | |
| - | + | <div style="background-color:#fffaf0;"> | |
| - | + | == Publication Abstract from PubMed == | |
| - | + | ||
| - | + | ||
| - | == | + | |
The structure of [113Cd(7)]-metallothionein (MT_nc) of the Antarctic fish Notothenia coriiceps, the first three-dimensional structure of a fish metallothionein, was determined by homonuclear 1H NMR experiments and heteronuclear [1H, 113Cd]-correlation spectroscopy. MT_nc is composed of an N-terminal beta domain with 9 cysteines and 3 metal ions and a carboxy-terminal alpha-domain with 11 cysteines and 4 metal ions. The position of the ninth Cys of the alpha domain of MT_nc is different from the corresponding Cys of mammalian MTs. As a result, the last CXCC motif in the mammalian MT sequence becomes CXXXCC in the fish MT. This difference leads to a structural change of the alpha domain and, in turn, to a different charge distribution with respect to that observed in mammalian metallothioneins. | The structure of [113Cd(7)]-metallothionein (MT_nc) of the Antarctic fish Notothenia coriiceps, the first three-dimensional structure of a fish metallothionein, was determined by homonuclear 1H NMR experiments and heteronuclear [1H, 113Cd]-correlation spectroscopy. MT_nc is composed of an N-terminal beta domain with 9 cysteines and 3 metal ions and a carboxy-terminal alpha-domain with 11 cysteines and 4 metal ions. The position of the ninth Cys of the alpha domain of MT_nc is different from the corresponding Cys of mammalian MTs. As a result, the last CXCC motif in the mammalian MT sequence becomes CXXXCC in the fish MT. This difference leads to a structural change of the alpha domain and, in turn, to a different charge distribution with respect to that observed in mammalian metallothioneins. | ||
| - | + | Solution structure of MT_nc, a novel metallothionein from the Antarctic fish Notothenia coriiceps.,Capasso C, Carginale V, Crescenzi O, Di Maro D, Parisi E, Spadaccini R, Temussi PA Structure. 2003 Apr;11(4):435-43. PMID:12679021<ref>PMID:12679021</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 1m0g" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Notothenia coriiceps]] | [[Category: Notothenia coriiceps]] | ||
| - | + | [[Category: Capasso C]] | |
| - | [[Category: Capasso | + | [[Category: Carginale V]] |
| - | [[Category: Carginale | + | [[Category: Crescenzi O]] |
| - | [[Category: Crescenzi | + | [[Category: Di Maro D]] |
| - | [[Category: Maro | + | [[Category: Parisi E]] |
| - | [[Category: Parisi | + | [[Category: Spadaccini R]] |
| - | [[Category: Spadaccini | + | [[Category: Temussi PA]] |
| - | [[Category: Temussi | + | |
| - | + | ||
| - | + | ||
| - | + | ||
Current revision
Solution structure of the alpha domain of mt_nc
| |||||||||||
