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4kha

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==Structural basis of histone H2A-H2B recognition by the essential chaperone FACT==
==Structural basis of histone H2A-H2B recognition by the essential chaperone FACT==
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<StructureSection load='4kha' size='340' side='right' caption='[[4kha]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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<StructureSection load='4kha' size='340' side='right'caption='[[4kha]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4kha]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KHA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KHA FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4kha]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495] and [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KHA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KHA FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTHT_0052370, CTHT_0052370 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog]), hist1h2aj, LOC494591 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kha OCA], [http://pdbe.org/4kha PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kha RCSB], [http://www.ebi.ac.uk/pdbsum/4kha PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kha ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kha OCA], [https://pdbe.org/4kha PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kha RCSB], [https://www.ebi.ac.uk/pdbsum/4kha PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kha ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/H2B11_XENLA H2B11_XENLA] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.[https://www.uniprot.org/uniprot/G0SDN1_CHATD G0SDN1_CHATD]
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Facilitates chromatin transcription (FACT) is a conserved histone chaperone that reorganizes nucleosomes and ensures chromatin integrity during DNA transcription, replication and repair. Key to the broad functions of FACT is its recognition of histones H2A-H2B (ref. 2). However, the structural basis for how histones H2A-H2B are recognized and how this integrates with the other functions of FACT, including the recognition of histones H3-H4 and other nuclear factors, is unknown. Here we reveal the crystal structure of the evolutionarily conserved FACT chaperone domain Spt16M from Chaetomium thermophilum, in complex with the H2A-H2B heterodimer. A novel 'U-turn' motif scaffolded onto a Rtt106-like module embraces the alpha1 helix of H2B. Biochemical and in vivo assays validate the structure and dissect the contribution of histone tails and H3-H4 towards Spt16M binding. Furthermore, we report the structure of the FACT heterodimerization domain that connects FACT to replicative polymerases. Our results show that Spt16M makes several interactions with histones, which we suggest allow the module to invade the nucleosome gradually and block the strongest interaction of H2B with DNA. FACT would thus enhance 'nucleosome breathing' by re-organizing the first 30 base pairs of nucleosomal histone-DNA contacts. Our snapshot of the engagement of the chaperone with H2A-H2B and the structures of all globular FACT domains enable the high-resolution analysis of the vital chaperoning functions of FACT, shedding light on how the complex promotes the activity of enzymes that require nucleosome reorganization.
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Structural basis of histone H2A-H2B recognition by the essential chaperone FACT.,Hondele M, Stuwe T, Hassler M, Halbach F, Bowman A, Zhang ET, Nijmeijer B, Kotthoff C, Rybin V, Amlacher S, Hurt E, Ladurner AG Nature. 2013 May 22. doi: 10.1038/nature12242. PMID:23698368<ref>PMID:23698368</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4kha" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: African clawed frog]]
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[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
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[[Category: Halbach, F]]
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[[Category: Large Structures]]
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[[Category: Hassler, M]]
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[[Category: Xenopus laevis]]
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[[Category: Hondele, M]]
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[[Category: Halbach F]]
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[[Category: Ladurner, A G]]
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[[Category: Hassler M]]
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[[Category: Chaperone-nuclear protein complex]]
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[[Category: Hondele M]]
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[[Category: Chromatin]]
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[[Category: Ladurner AG]]
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[[Category: Histone chaperone]]
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[[Category: Histone]]
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[[Category: Nucleus]]
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[[Category: Pleckstrin-homology like]]
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[[Category: Tandem phl]]
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[[Category: Transcription]]
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[[Category: U-turn motif]]
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Current revision

Structural basis of histone H2A-H2B recognition by the essential chaperone FACT

PDB ID 4kha

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