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| | ==Crystal structure of H107R mutant of extracellular domain of mouse receptor NKR-P1A== | | ==Crystal structure of H107R mutant of extracellular domain of mouse receptor NKR-P1A== |
| - | <StructureSection load='3t3a' size='340' side='right' caption='[[3t3a]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='3t3a' size='340' side='right'caption='[[3t3a]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3t3a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T3A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T3A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3t3a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T3A FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3m9z|3m9z]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Klrb1a, Ly55, Ly55a, Nkrp1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t3a OCA], [https://pdbe.org/3t3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t3a RCSB], [https://www.ebi.ac.uk/pdbsum/3t3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t3a ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t3a OCA], [http://pdbe.org/3t3a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t3a RCSB], [http://www.ebi.ac.uk/pdbsum/3t3a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t3a ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/KLRBA_MOUSE KLRBA_MOUSE]] Plays a stimulatory role on natural killer (NK) cell cytotoxicity.<ref>PMID:16751398</ref> | + | [https://www.uniprot.org/uniprot/KLRBA_MOUSE KLRBA_MOUSE] Plays a stimulatory role on natural killer (NK) cell cytotoxicity.<ref>PMID:16751398</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Bezouska, K]] | + | [[Category: Mus musculus]] |
| - | [[Category: Dohnalek, J]] | + | [[Category: Bezouska K]] |
| - | [[Category: Hasek, J]] | + | [[Category: Dohnalek J]] |
| - | [[Category: Kolenko, P]] | + | [[Category: Hasek J]] |
| - | [[Category: Rozbesky, D]] | + | [[Category: Kolenko P]] |
| - | [[Category: C-type lectin-like domain]]
| + | [[Category: Rozbesky D]] |
| - | [[Category: Domain swapping]]
| + | |
| - | [[Category: Natural killer cell receptor]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| - | [[Category: Transmembrane receptor]]
| + | |
| - | [[Category: Twinning]]
| + | |
| Structural highlights
Function
KLRBA_MOUSE Plays a stimulatory role on natural killer (NK) cell cytotoxicity.[1]
Publication Abstract from PubMed
The structure of the H107R variant of the extracellular domain of the mouse natural killer cell receptor NKR-P1A has been determined by X-ray diffraction at 2.3 A resolution from a merohedrally twinned crystal. Unlike the structure of the wild-type receptor in space group I4(1)22 with a single chain per asymmetric unit, the crystals of the variant belonged to space group I4(1) with a dimer in the asymmetric unit. Different degrees of merohedral twinning were detected in five data sets collected from different crystals. The mutation does not have a significant impact on the overall structure, but led to the binding of an additional phosphate ion at the interface of the molecules.
Structure of the H107R variant of the extracellular domain of mouse NKR-P1A at 2.3 A resolution.,Kolenko P, Rozbesky D, Vanek O, Bezouska K, Hasek J, Dohnalek J Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt 12):1519-23., Epub 2011 Nov 30. PMID:22139156[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Carlyle JR, Mesci A, Ljutic B, Belanger S, Tai LH, Rousselle E, Troke AD, Proteau MF, Makrigiannis AP. Molecular and genetic basis for strain-dependent NK1.1 alloreactivity of mouse NK cells. J Immunol. 2006 Jun 15;176(12):7511-24. PMID:16751398
- ↑ Kolenko P, Rozbesky D, Vanek O, Bezouska K, Hasek J, Dohnalek J. Structure of the H107R variant of the extracellular domain of mouse NKR-P1A at 2.3 A resolution. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt 12):1519-23., Epub 2011 Nov 30. PMID:22139156 doi:10.1107/S1744309111046203
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