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| | ==Crystal structure of TTC0807 complexed with Ornithine== | | ==Crystal structure of TTC0807 complexed with Ornithine== |
| - | <StructureSection load='3vvd' size='340' side='right' caption='[[3vvd]], [[Resolution|resolution]] 2.05Å' scene=''> | + | <StructureSection load='3vvd' size='340' side='right'caption='[[3vvd]], [[Resolution|resolution]] 2.05Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3vvd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet2 Thet2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VVD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VVD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3vvd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VVD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VVD FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vve|3vve]], [[3vvf|3vvf]], [[3vv5|3vv5]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TTC0807, TT_C0807 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 THET2])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vvd OCA], [https://pdbe.org/3vvd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vvd RCSB], [https://www.ebi.ac.uk/pdbsum/3vvd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vvd ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vvd OCA], [http://pdbe.org/3vvd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vvd RCSB], [http://www.ebi.ac.uk/pdbsum/3vvd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vvd ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q72JG5_THET2 Q72JG5_THET2] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Thet2]] | + | [[Category: Large Structures]] |
| - | [[Category: Hasebe, F]] | + | [[Category: Thermus thermophilus HB27]] |
| - | [[Category: Kanemaru, Y]] | + | [[Category: Hasebe F]] |
| - | [[Category: Kuzuyama, T]] | + | [[Category: Kanemaru Y]] |
| - | [[Category: Nishiyama, M]] | + | [[Category: Kuzuyama T]] |
| - | [[Category: Tomita, T]] | + | [[Category: Nishiyama M]] |
| - | [[Category: Ornithine-complex]]
| + | [[Category: Tomita T]] |
| - | [[Category: Periplasm substrate binding protein]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
Q72JG5_THET2
Publication Abstract from PubMed
Thermus thermophilus exhibits hypersensitivity to a lysine analog, (S)-2-aminoethyl-cysteine (AEC). Cosmid libraries were constructed using genomes from two AEC-resistant mutants, AT10 and AT14, and the cosmids that conferred AEC resistance on the wild-type strain were isolated. When the cosmid library for mutant AT14 was screened, two independent cosmids, conferring partial AEC resistance to the wild type, were obtained. Two cosmids carried a common genomic region from TTC0795 to TTC0810. This region contains genes encoding an ATP-binding cassette (ABC) transporter consisting of TTC0806/TTC0795, using TTC0807 as the periplasmic substrate-binding protein. Sequencing revealed that AT14 carries mutations in TTC0795 and TTC0969, causing decreases in the thermostability of the products. TTC0969 encodes the nucleotide-binding protein of a different ABC transporter consisting of TTC0967/TTC0968/TTC0969/TTC0970 using TTC0966 as the periplasmic substrate-binding protein. By similar screening for cosmids constructed for the mutant AT10, mutations were found at TTC0807 and TTC0969. Mutation in either of the transporter components gave partial resistance to AEC in the wild-type strain, while mutations of both transporters conferred complete AEC resistance. This result indicates that both transporters are involved in AEC uptake in T. thermophilus. To elucidate the mechanism of AEC uptake, crystal structures of TTC0807 were determined in several substrate-binding forms. The structures revealed that TTC0807 recognizes various basic amino acids by changing the side-chain conformation of Glu19, which interacts with the side-chain amino groups of the substrates.
Two ATP-Binding Cassette Transporters Involved in (S)-2-Aminoethyl-Cysteine Uptake in Thermus thermophilus.,Kanemaru Y, Hasebe F, Tomita T, Kuzuyama T, Nishiyama M J Bacteriol. 2013 Sep;195(17):3845-53. doi: 10.1128/JB.00202-13. Epub 2013 Jun, 21. PMID:23794618[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kanemaru Y, Hasebe F, Tomita T, Kuzuyama T, Nishiyama M. Two ATP-Binding Cassette Transporters Involved in (S)-2-Aminoethyl-Cysteine Uptake in Thermus thermophilus. J Bacteriol. 2013 Sep;195(17):3845-53. doi: 10.1128/JB.00202-13. Epub 2013 Jun, 21. PMID:23794618 doi:10.1128/JB.00202-13
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