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| | ==Crystal structure of P. falciparum Lysyl-tRNA synthetase== | | ==Crystal structure of P. falciparum Lysyl-tRNA synthetase== |
| - | <StructureSection load='4h02' size='340' side='right' caption='[[4h02]], [[Resolution|resolution]] 2.91Å' scene=''> | + | <StructureSection load='4h02' size='340' side='right'caption='[[4h02]], [[Resolution|resolution]] 2.91Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4h02]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Plaf7 Plaf7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H02 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4h02]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H02 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bju|3bju]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.905Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF13_0262 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h02 OCA], [https://pdbe.org/4h02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h02 RCSB], [https://www.ebi.ac.uk/pdbsum/4h02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h02 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysine--tRNA_ligase Lysine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.6 6.1.1.6] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h02 OCA], [http://pdbe.org/4h02 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h02 RCSB], [http://www.ebi.ac.uk/pdbsum/4h02 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h02 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q8IDJ8_PLAF7 Q8IDJ8_PLAF7] |
| - | Aminoacyl-tRNA synthetases are essential enzymes that transmit information from the genetic code to proteins in cells and are targets for antipathogen drug development. Elucidation of the crystal structure of cytoplasmic lysyl-tRNA synthetase from the malaria parasite Plasmodium falciparum (PfLysRS) has allowed direct comparison with human LysRS. The authors' data suggest that PfLysRS is dimeric in solution, whereas the human counterpart can also adopt tetrameric forms. It is shown for the first time that PfLysRS is capable of synthesizing the signalling molecule Ap4a (diadenosine tetraphosphate) using ATP as a substrate. The PfLysRS crystal structure is in the apo form, such that binding to ATP will require rotameric changes in four conserved residues. Differences in the active-site regions of parasite and human LysRSs suggest the possibility of exploiting PfLysRS for selective inhibition. These investigations on PfLysRS further validate malarial LysRSs as attractive antimalarial targets and provide new structural space for the development of inhibitors that target pathogen LysRSs selectively.
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| - | Structural analysis of malaria-parasite lysyl-tRNA synthetase provides a platform for drug development.,Khan S, Garg A, Camacho N, Van Rooyen J, Kumar Pole A, Belrhali H, Ribas de Pouplana L, Sharma V, Sharma A Acta Crystallogr D Biol Crystallogr. 2013 May;69(Pt 5):785-95. doi:, 10.1107/S0907444913001923. Epub 2013 Apr 11. PMID:23633587<ref>PMID:23633587</ref>
| + | ==See Also== |
| - | | + | *[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 4h02" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lysine--tRNA ligase]] | + | [[Category: Large Structures]] |
| - | [[Category: Plaf7]] | + | [[Category: Plasmodium falciparum 3D7]] |
| - | [[Category: Garg, A]] | + | [[Category: Garg A]] |
| - | [[Category: Khan, S]] | + | [[Category: Khan S]] |
| - | [[Category: Sharma, A]] | + | [[Category: Sharma A]] |
| - | [[Category: Cladosporin]]
| + | |
| - | [[Category: Ligase]]
| + | |
| - | [[Category: Malaria]]
| + | |
