|
|
| (2 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal Structure of a dimeric variant of Bet v 1== | | ==Crystal Structure of a dimeric variant of Bet v 1== |
| - | <StructureSection load='4bk6' size='340' side='right' caption='[[4bk6]], [[Resolution|resolution]] 1.63Å' scene=''> | + | <StructureSection load='4bk6' size='340' side='right'caption='[[4bk6]], [[Resolution|resolution]] 1.63Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4bk6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Betpn Betpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BK6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BK6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4bk6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Betula_pendula Betula pendula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BK6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BK6 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bk7|4bk7]], [[4bkc|4bkc]], [[4bkd|4bkd]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bk6 OCA], [http://pdbe.org/4bk6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bk6 RCSB], [http://www.ebi.ac.uk/pdbsum/4bk6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bk6 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bk6 OCA], [https://pdbe.org/4bk6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bk6 RCSB], [https://www.ebi.ac.uk/pdbsum/4bk6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bk6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BEV1A_BETPN BEV1A_BETPN]] May be a general steroid carrier protein (By similarity). | + | [https://www.uniprot.org/uniprot/BEV1A_BETPN BEV1A_BETPN] May be a general steroid carrier protein (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Betpn]] | + | [[Category: Betula pendula]] |
| - | [[Category: Brandstetter, H]] | + | [[Category: Large Structures]] |
| - | [[Category: Kofler, S G]] | + | [[Category: Brandstetter H]] |
| - | [[Category: Allergen]] | + | [[Category: Kofler SG]] |
| Structural highlights
Function
BEV1A_BETPN May be a general steroid carrier protein (By similarity).
Publication Abstract from PubMed
Many allergens share several biophysical characteristics, including the capability to undergo oligomerisation. The dimerization mechanism in Bet v 1 and its allergenic properties are so far poorly understood. Here, we report crystal structures of dimeric Bet v 1, revealing a non-canonical incorporation of cysteine at position 5 instead of genetically encoded tyrosine. Cysteine polysulfide bridging stabilized different dimeric assemblies, depending on the polysulfide linker length. These dimers represent quaternary arrangements that are frequently observed in related proteins, reflecting their prevalence in unmodified Bet v 1. These conclusions were corroborated by characteristic immunologic properties of monomeric and dimeric allergen variants. Hereby, residue 5 could be identified as an allergenic hot spot in Bet v 1. The presented results refine fundamental principles in protein chemistry, and emphasize the importance of protein modifications in understanding the molecular basis of allergenicity.
Stabilization of the dimeric birch pollen allergen Bet v 1 impacts its immunological properties.,Kofler S, Ackaert C, Samonig M, Asam C, Briza P, Horejs-Hoeck J, Cabrele C, Ferreira F, Duschl A, Huber C, Brandstetter H J Biol Chem. 2013 Nov 19. PMID:24253036[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kofler S, Ackaert C, Samonig M, Asam C, Briza P, Horejs-Hoeck J, Cabrele C, Ferreira F, Duschl A, Huber C, Brandstetter H. Stabilization of the dimeric birch pollen allergen Bet v 1 impacts its immunological properties. J Biol Chem. 2013 Nov 19. PMID:24253036 doi:http://dx.doi.org/10.1074/jbc.M113.518795
|
