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| | ==beta-tryptase inhibitor== | | ==beta-tryptase inhibitor== |
| - | <StructureSection load='4a6l' size='340' side='right' caption='[[4a6l]], [[Resolution|resolution]] 2.05Å' scene=''> | + | <StructureSection load='4a6l' size='340' side='right'caption='[[4a6l]], [[Resolution|resolution]] 2.05Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4a6l]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A6L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A6L FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4a6l]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A6L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A6L FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P43:1-{3-[1-({5-[(2-FLUOROPHENYL)ETHYNYL]FURAN-2-YL}CARBONYL)PIPERIDIN-4-YL]PHENYL}METHANAMINE'>P43</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1aao|1aao]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P43:1-{3-[1-({5-[(2-FLUOROPHENYL)ETHYNYL]FURAN-2-YL}CARBONYL)PIPERIDIN-4-YL]PHENYL}METHANAMINE'>P43</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptase Tryptase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.59 3.4.21.59] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a6l OCA], [https://pdbe.org/4a6l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a6l RCSB], [https://www.ebi.ac.uk/pdbsum/4a6l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a6l ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a6l OCA], [http://pdbe.org/4a6l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a6l RCSB], [http://www.ebi.ac.uk/pdbsum/4a6l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a6l ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TRYB1_HUMAN TRYB1_HUMAN]] Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type (By similarity). | + | [https://www.uniprot.org/uniprot/TRYB2_HUMAN TRYB2_HUMAN] Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Tryptase]] | + | [[Category: Large Structures]] |
| - | [[Category: Maignan, S]] | + | [[Category: Maignan S]] |
| - | [[Category: Mathieu, M]] | + | [[Category: Mathieu M]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Inhibitor]]
| + | |
| Structural highlights
Function
TRYB2_HUMAN Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection (By similarity).
Publication Abstract from PubMed
A solid phase combinatorial library was designed based on X-ray structures and in-silico models to explore an inducible S4+ pocket, which is formed by a simple side-chain rotation of Tyr95. This inducible S4+ pocket is unique to beta-tryptase and does not exist for other trypsin-like serine proteases of interest. Therefore, inhibitors utilizing this pocket have inherent advantages for being selective against other proteases in the same family. A member of this library was found to be a potent and selective beta-tryptase inhibitor with a suitable pharmacokinetic profile for further clinical evaluation.
Structure-based library design and the discovery of a potent and selective mast cell beta-tryptase inhibitor as an oral therapeutic agent.,Liang G, Aldous S, Merriman G, Levell J, Pribish J, Cairns J, Chen X, Maignan S, Mathieu M, Tsay J, Sides K, Rebello S, Whitely B, Morize I, Pauls HW Bioorg Med Chem Lett. 2012 Jan 15;22(2):1049-54. Epub 2011 Dec 7. PMID:22192588[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Liang G, Aldous S, Merriman G, Levell J, Pribish J, Cairns J, Chen X, Maignan S, Mathieu M, Tsay J, Sides K, Rebello S, Whitely B, Morize I, Pauls HW. Structure-based library design and the discovery of a potent and selective mast cell beta-tryptase inhibitor as an oral therapeutic agent. Bioorg Med Chem Lett. 2012 Jan 15;22(2):1049-54. Epub 2011 Dec 7. PMID:22192588 doi:10.1016/j.bmcl.2011.11.119
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