3pgg

<StructureSection load='3pgg' size='340' side='right' caption='[[3pgg]], [[Resolution|resolution]] 2.14&Aring;' scene=''>

<table><tr><td colspan='2'>[[3pgg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Crypv Crypv]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2fwk 2fwk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PGG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PGG FirstGlance]. <br>

</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cgd7_4580 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5807 CRYPV])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pgg OCA], [http://pdbe.org/3pgg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pgg RCSB], [http://www.ebi.ac.uk/pdbsum/3pgg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pgg ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Parasites from the protozoan phylum Apicomplexa are responsible for diseases, such as malaria, toxoplasmosis and cryptosporidiosis, all of which have significantly higher rates of mortality and morbidity in economically underdeveloped regions of the world. Advances in vaccine development and drug discovery are urgently needed to control these diseases and can be facilitated by production of purified recombinant proteins from Apicomplexan genomes and determination of their 3D structures. To date, both heterologous expression and crystallization of Apicomplexan proteins have seen only limited success. In an effort to explore the effectiveness of producing and crystallizing proteins on a genome-scale using a standardized methodology, over 400 distinct Plasmodium falciparum target genes were chosen representing different cellular classes, along with select orthologues from four other Plasmodium species as well as Cryptosporidium parvum and Toxoplasma gondii. From a total of 1008 genes from the seven genomes, 304 (30.2%) produced purified soluble proteins and 97 (9.6%) crystallized, culminating in 36 crystal structures. These results demonstrate that, contrary to previous findings, a standardized platform using Escherichia coli can be effective for genome-scale production and crystallography of Apicomplexan proteins. Predictably, orthologous proteins from different Apicomplexan genomes behaved differently in expression, purification and crystallization, although the overall success rates of Plasmodium orthologues do not differ significantly. Their differences were effectively exploited to elevate the overall productivity to levels comparable to the most successful ongoing structural genomics projects: 229 of the 468 target genes produced purified soluble protein from one or more organisms, with 80 and 32 of the purified targets, respectively, leading to crystals and ultimately structures from one or more orthologues.

 

Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms.,Vedadi M, Lew J, Artz J, Amani M, Zhao Y, Dong A, Wasney GA, Gao M, Hills T, Brokx S, Qiu W, Sharma S, Diassiti A, Alam Z, Melone M, Mulichak A, Wernimont A, Bray J, Loppnau P, Plotnikova O, Newberry K, Sundararajan E, Houston S, Walker J, Tempel W, Bochkarev A, Kozieradzki I, Edwards A, Arrowsmith C, Roos D, Kain K, Hui R Mol Biochem Parasitol. 2007 Jan;151(1):100-10. Epub 2006 Nov 13. PMID:17125854<ref>PMID:17125854</ref>

 

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 3pgg" style="background-color:#fffaf0;"></div>

*[[Sm-like protein|Sm-like protein]]

[[Category: Crypv]]

[[Category: Arrowsmith, C]]

[[Category: Artz, J]]

[[Category: Bochkarev, A]]

[[Category: Dong, A]]

[[Category: Edwards, A]]

[[Category: Gao, M]]

[[Category: Hui, R]]

[[Category: Kozieradzki, I]]

[[Category: Lew, J]]

[[Category: Structural genomic]]

[[Category: Sundstrom, M]]

[[Category: Vedadi, M]]

[[Category: Wasney, G A]]

[[Category: Weigelt, J]]

[[Category: Zhao, Y]]

[[Category: U6 snrna-associated sm-like protein]]