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| | ==Structual basis for the recognition of Ubc13 by the Shigella flexneri effector OspI== | | ==Structual basis for the recognition of Ubc13 by the Shigella flexneri effector OspI== |
| - | <StructureSection load='3w30' size='340' side='right' caption='[[3w30]], [[Resolution|resolution]] 2.99Å' scene=''> | + | <StructureSection load='3w30' size='340' side='right'caption='[[3w30]], [[Resolution|resolution]] 2.99Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3w30]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W30 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W30 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3w30]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W30 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3W30 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3w31|3w31]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.99Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ORF169b, CP0209 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3w30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w30 OCA], [https://pdbe.org/3w30 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3w30 RCSB], [https://www.ebi.ac.uk/pdbsum/3w30 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3w30 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w30 OCA], [http://pdbe.org/3w30 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3w30 RCSB], [http://www.ebi.ac.uk/pdbsum/3w30 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3w30 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8VSD5_SHIFL Q8VSD5_SHIFL] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Shigella paradysenteriae weldin 1927]] | + | [[Category: Large Structures]] |
| - | [[Category: Kim, M]] | + | [[Category: Shigella flexneri]] |
| - | [[Category: Mizushima, T]] | + | [[Category: Kim M]] |
| - | [[Category: Nishide, A]] | + | [[Category: Mizushima T]] |
| - | [[Category: Sasakawa, C]] | + | [[Category: Nishide A]] |
| - | [[Category: Takagi, K]] | + | [[Category: Sasakawa C]] |
| - | [[Category: Deamidation]]
| + | [[Category: Takagi K]] |
| - | [[Category: Effector]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Type 3 secretion system]]
| + | |
| Structural highlights
Function
Q8VSD5_SHIFL
Publication Abstract from PubMed
Ubc13 is a ubiquitin-conjugating enzyme that plays a key role in the nuclear factor-kappaB signal transduction pathway in human diseases. The Shigella flexneri effector OspI affects inflammatory responses by catalyzing the deamidation of a specific glutamine residue at position 100 in Ubc13 during infection. This modification prevents the activation of the TNF (tumor necrosis factor) receptor-associated factor 6, leading to modulation of the diacylglycerol-CBM (CARD-Bcl10-Malt1) complex-TNF receptor-associated factor 6-nuclear factor-kappaB signaling pathway. To elucidate the structural basis of OspI function, we determined the crystal structures of the catalytically inert OspI C62A mutant and its complex with Ubc13 at resolutions of 3.0 and 2.96A, respectively. The structure of the OspI-Ubc13 complex revealed that the interacting surfaces between OspI and Ubc13 are a hydrophobic surface and a complementary charged surface. Furthermore, we predict that the complementary charged surface of OspI plays a key role in substrate specificity determination.
Structural Basis for the Recognition of Ubc13 by the Shigella flexneri Effector OspI.,Nishide A, Kim M, Takagi K, Himeno A, Sanada T, Sasakawa C, Mizushima T J Mol Biol. 2013 Aug 9;425(15):2623-31. doi: 10.1016/j.jmb.2013.02.037. Epub 2013, Mar 27. PMID:23542009[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Nishide A, Kim M, Takagi K, Himeno A, Sanada T, Sasakawa C, Mizushima T. Structural Basis for the Recognition of Ubc13 by the Shigella flexneri Effector OspI. J Mol Biol. 2013 Aug 9;425(15):2623-31. doi: 10.1016/j.jmb.2013.02.037. Epub 2013, Mar 27. PMID:23542009 doi:10.1016/j.jmb.2013.02.037
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