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| | ==Crystal Structure of Htt36Q3H-EX1-X1-C1(Alpha)== | | ==Crystal Structure of Htt36Q3H-EX1-X1-C1(Alpha)== |
| - | <StructureSection load='4fe8' size='340' side='right' caption='[[4fe8]], [[Resolution|resolution]] 3.00Å' scene=''> | + | <StructureSection load='4fe8' size='340' side='right'caption='[[4fe8]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4fe8]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FE8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FE8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4fe8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FE8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FE8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3io4|3io4]], [[3io6|3io6]], [[3iot|3iot]], [[3iou|3iou]], [[3ior|3ior]], [[3iov|3iov]], [[3iow|3iow]], [[4feb|4feb]], [[4fec|4fec]], [[4fed|4fed]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">malE, b4034, JW3994, HD, HTT, IT15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fe8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fe8 OCA], [https://pdbe.org/4fe8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fe8 RCSB], [https://www.ebi.ac.uk/pdbsum/4fe8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fe8 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fe8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fe8 OCA], [http://pdbe.org/4fe8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fe8 RCSB], [http://www.ebi.ac.uk/pdbsum/4fe8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fe8 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Disease == |
| | + | [https://www.uniprot.org/uniprot/HD_HUMAN HD_HUMAN] Juvenile Huntington disease;Huntington disease. The disease is caused by mutations affecting the gene represented in this entry. |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides. | + | [https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/HD_HUMAN HD_HUMAN] May play a role in microtubule-mediated transport or vesicle function. |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Huntington disease is an autosomal-dominant neurodegenerative disorder caused by a polyglutamine (polyQ) expansion (> 35Q) in the first exon (EX1) of huntingtin protein (Htt). mHtt protein is thought to adopt one or more toxic conformation(s) that are involved in pathogenic interactions in cells . However, the structure of mHtt is not known. Here, we present a near atomic resolution structure of mHtt36Q-EX1. To facilitate crystallization, three histidine residues (3H) were introduced within the Htt36Q stretch resulting in the sequence of Q 7HQHQHQ 27. The Htt36Q3H region adopts alpha-helix, loop, beta-hairpin conformations. Furthermore, we observed interactions between the backbone of the Htt36Q3H beta-strand with the aromatic residues mimicking putative-toxic interactions with other proteins. Our findings support previous predictions that the expanded mHtt-polyQ region adopts a beta-sheet structure. Detailed structural information about mHtt improves our understanding of the pathogenic mechanisms in HD and other polyQ expansion disorders and may form the basis for rational design of small molecules that target toxic conformations of disease-causing proteins.
| + | |
| | | | |
| - | Beta conformation of polyglutamine track revealed by a crystal structure of Huntingtin N-terminal region with insertion of three histidine residues.,Kim M Prion. 2013 Jan 31;7(3). PMID:23370273<ref>PMID:23370273</ref>
| + | ==See Also== |
| - | | + | *[[Huntingtin|Huntingtin]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 4fe8" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Kim, M]]
| + | [[Category: Homo sapiens]] |
| - | [[Category: Alpha helix]]
| + | [[Category: Large Structures]] |
| - | [[Category: Beta strand hairpin]]
| + | [[Category: Kim M]] |
| - | [[Category: Beta-strand hairpin]]
| + | |
| - | [[Category: Disease protein]] | + | |
| - | [[Category: Loop]] | + | |
| - | [[Category: Signaling protein]] | + | |
