This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2ya1

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Product complex of a multi-modular glycogen-degrading pneumococcal virulence factor SpuA

Structural highlights

2ya1 is a 1 chain structure with sequence from Streptococcus pneumoniae TIGR4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.25Å
Ligands:	, , , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PULA_STRPN Virulence factor (By similarity). Involved in the degradation of glycogen of the mammalian host cells (PubMed:21565699). Hydrolyzes the alpha-1,6-branchpoints of glycogen (PubMed:20497336, PubMed:21565699). Hydrolyzes pullulan. Does not hydrolyze dextran (PubMed:20497336). Binds to mouse lung alveolar type II cells that are rich in glycogen stores. Is an alpha-glucan-specific carbohydrate-binding protein, which binds to amylose (pure alpha-(1,4)-linked glucose), amylopectin (alpha-(1,4)-linked glucose with alpha-(1,6) branch points), pullulan (linear polymer of mixed alpha-(1,4)- and alpha-(1,6)-linked glucose) and glycogen (similar to amylopectin with more frequent alpha-(1,6) branch points) in vitro. Does not bind to dextran (a linear polymer of alpha-(1,6)-linked glucose) (PubMed:17187076).[UniProtKB:A0A0H2ZL64]^[1] ^[2] ^[3]

Publication Abstract from PubMed

SpuA is a large multimodular cell wall-attached enzyme involved in the degradation of glycogen by the pathogenic bacterium Streptococcus pneumoniae. The deletion of the gene encoding SpuA from the bacterium resulted in a strain with reduced competitiveness in a mouse model of virulence relative to the parent strain, linking the degradation of host-glycogen to the virulence of the bacterium. Through the combined use of X-ray crystallography, small-angle X-ray scattering, and inhibitor binding, the molecular features involved in substrate recognition by this complex protein are revealed. This uniquely illustrates the complexity of the active site, the conformational changes incurred during carbohydrate binding by this protein, and the interaction and cooperation of its composite modules during this process. New insight into the function of this particular pneumococcal virulence factor is provided along with substantial contributions to the nascent framework for understanding the structural and functional interplay between modules in multimodular carbohydrate-active enzymes.

The conformation and function of a multimodular glycogen-degrading pneumococcal virulence factor.,Lammerts van Bueren A, Ficko-Blean E, Pluvinage B, Hehemann JH, Higgins MA, Deng L, Ogunniyi AD, Stroeher UH, El Warry N, Burke RD, Czjzek M, Paton JC, Vocadlo DJ, Boraston AB Structure. 2011 May 11;19(5):640-51. PMID:21565699^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ van Bueren AL, Higgins M, Wang D, Burke RD, Boraston AB. Identification and structural basis of binding to host lung glycogen by streptococcal virulence factors. Nat Struct Mol Biol. 2007 Jan;14(1):76-84. Epub 2006 Dec 24. PMID:17187076 doi:http://dx.doi.org/10.1038/nsmb1187
↑ Abbott DW, Higgins MA, Hyrnuik S, Pluvinage B, Lammerts van Bueren A, Boraston AB. The molecular basis of glycogen breakdown and transport in Streptococcus pneumoniae. Mol Microbiol. 2010 Jul 1;77(1):183-99. Epub 2010 May 19. PMID:20497336 doi:10.1111/j.1365-2958.2010.07199.x
↑ Lammerts van Bueren A, Ficko-Blean E, Pluvinage B, Hehemann JH, Higgins MA, Deng L, Ogunniyi AD, Stroeher UH, El Warry N, Burke RD, Czjzek M, Paton JC, Vocadlo DJ, Boraston AB. The conformation and function of a multimodular glycogen-degrading pneumococcal virulence factor. Structure. 2011 May 11;19(5):640-51. PMID:21565699 doi:10.1016/j.str.2011.03.001
↑ Lammerts van Bueren A, Ficko-Blean E, Pluvinage B, Hehemann JH, Higgins MA, Deng L, Ogunniyi AD, Stroeher UH, El Warry N, Burke RD, Czjzek M, Paton JC, Vocadlo DJ, Boraston AB. The conformation and function of a multimodular glycogen-degrading pneumococcal virulence factor. Structure. 2011 May 11;19(5):640-51. PMID:21565699 doi:10.1016/j.str.2011.03.001

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ya1"

@@ Line 1: / Line 1: @@
 ==Product complex of a multi-modular glycogen-degrading pneumococcal virulence factor SpuA==
-<StructureSection load='2ya1' size='340' side='right' caption='[[2ya1]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
+<StructureSection load='2ya1' size='340' side='right'caption='[[2ya1]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2ya1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YA1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2ya1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YA1 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ya0|2ya0]], [[2j44|2j44]], [[2ya2|2ya2]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PRD_900009:alpha-maltotriose'>PRD_900009</scene>, <scene name='pdbligand=PRD_900010:alpha-maltotetraose'>PRD_900010</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ya1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ya1 OCA], [http://pdbe.org/2ya1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ya1 RCSB], [http://www.ebi.ac.uk/pdbsum/2ya1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ya1 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ya1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ya1 OCA], [https://pdbe.org/2ya1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ya1 RCSB], [https://www.ebi.ac.uk/pdbsum/2ya1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ya1 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/PULA_STRPN PULA_STRPN] Virulence factor (By similarity). Involved in the degradation of glycogen of the mammalian host cells (PubMed:21565699). Hydrolyzes the alpha-1,6-branchpoints of glycogen (PubMed:20497336, PubMed:21565699). Hydrolyzes pullulan. Does not hydrolyze dextran (PubMed:20497336). Binds to mouse lung alveolar type II cells that are rich in glycogen stores. Is an alpha-glucan-specific carbohydrate-binding protein, which binds to amylose (pure alpha-(1,4)-linked glucose), amylopectin (alpha-(1,4)-linked glucose with alpha-(1,6) branch points), pullulan (linear polymer of mixed alpha-(1,4)- and alpha-(1,6)-linked glucose) and glycogen (similar to amylopectin with more frequent alpha-(1,6) branch points) in vitro. Does not bind to dextran (a linear polymer of alpha-(1,6)-linked glucose) (PubMed:17187076).[UniProtKB:A0A0H2ZL64]<ref>PMID:17187076</ref> <ref>PMID:20497336</ref> <ref>PMID:21565699</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Strpn]]
+[[Category: Large Structures]]
-[[Category: Boraston, A B]]
+[[Category: Streptococcus pneumoniae TIGR4]]
-[[Category: Bueren, A Lammerts van]]
+[[Category: Boraston AB]]
-[[Category: Burke, R D]]
+[[Category: Burke RD]]
-[[Category: Czjzek, M]]
+[[Category: Czjzek M]]
-[[Category: Deng, L]]
+[[Category: Deng L]]
-[[Category: Ficko-Blean, E]]
+[[Category: Ficko-Blean E]]
-[[Category: Hehemann, J H]]
+[[Category: Hehemann JH]]
-[[Category: Higgins, M A]]
+[[Category: Higgins MA]]
-[[Category: Ogunniyi, A D]]
+[[Category: Lammerts van Bueren A]]
-[[Category: Paton, J C]]
+[[Category: Ogunniyi AD]]
-[[Category: Pluvinage, B]]
+[[Category: Paton JC]]
-[[Category: Stroeher, U H]]
+[[Category: Pluvinage B]]
-[[Category: Vocadlo, D J]]
+[[Category: Stroeher UH]]
-[[Category: Warry, N E]]
+[[Category: Vocadlo DJ]]
-[[Category: Glycoside hydrolase]]
+[[Category: Warry NE]]
-[[Category: Hydrolase]]

2ya1

From Proteopedia

Current revision

Product complex of a multi-modular glycogen-degrading pneumococcal virulence factor SpuA

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox