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| | ==Rv2966c of M. tuberculosis is a RsmD-like methyltransferase== | | ==Rv2966c of M. tuberculosis is a RsmD-like methyltransferase== |
| - | <StructureSection load='3p9n' size='340' side='right' caption='[[3p9n]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='3p9n' size='340' side='right'caption='[[3p9n]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3p9n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P9N FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3p9n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P9N FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MT3044, Rv2966c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p9n OCA], [http://pdbe.org/3p9n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3p9n RCSB], [http://www.ebi.ac.uk/pdbsum/3p9n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3p9n ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p9n OCA], [https://pdbe.org/3p9n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p9n RCSB], [https://www.ebi.ac.uk/pdbsum/3p9n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p9n ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/P95128_MYCTO P95128_MYCTO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Myctu]] | + | [[Category: Large Structures]] |
| - | [[Category: Kumar, A]] | + | [[Category: Mycobacterium tuberculosis H37Rv]] |
| - | [[Category: Malhotra, K]] | + | [[Category: Kumar A]] |
| - | [[Category: Saigal, K]] | + | [[Category: Malhotra K]] |
| - | [[Category: Sinha, K M]] | + | [[Category: Saigal K]] |
| - | [[Category: Taneja, B]] | + | [[Category: Sinha KM]] |
| - | [[Category: Adomet binding]]
| + | [[Category: Taneja B]] |
| - | [[Category: Rna methylase]]
| + | |
| - | [[Category: Rna methyltransferase]]
| + | |
| - | [[Category: Rsmd]]
| + | |
| - | [[Category: Rv2966c]]
| + | |
| - | [[Category: Sam-fold]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
P95128_MYCTO
Publication Abstract from PubMed
Nine of ten methylated nucleotides of Escherichia coli 16 S rRNA are conserved in Mycobacterium tuberculosis. All the 10 different methyltransferases are known in E. coli, whereas only TlyA and GidB have been identified in mycobacteria. Here we have identified Rv2966c of M. tuberculosis as an ortholog of RsmD protein of E. coli. We have shown that rv2966c can complement rsmD-deleted E. coli cells. Recombinant Rv2966c can use 30 S ribosomes purified from rsmD-deleted E. coli as substrate and methylate G966 of 16 S rRNA in vitro. Structure determination of the protein shows the protein to be a two-domain structure with a short hairpin domain at the N terminus and a C-terminal domain with the S-adenosylmethionine-MT-fold. We show that the N-terminal hairpin is a minimalist functional domain that helps Rv2966c in target recognition. Deletion of the N-terminal domain prevents binding to nucleic acid substrates, and the truncated protein fails to carry out the m(2)G966 methylation on 16 S rRNA. The N-terminal domain also binds DNA efficiently, a property that may be utilized under specific conditions of cellular growth.
Structural and functional characterization of Rv2966c protein reveals an RsmD-like methyltransferase from Mycobacterium tuberculosis and the role of its N-terminal domain in target recognition.,Kumar A, Saigal K, Malhotra K, Sinha KM, Taneja B J Biol Chem. 2011 Jun 3;286(22):19652-61. Epub 2011 Apr 7. PMID:21474448[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kumar A, Saigal K, Malhotra K, Sinha KM, Taneja B. Structural and functional characterization of Rv2966c protein reveals an RsmD-like methyltransferase from Mycobacterium tuberculosis and the role of its N-terminal domain in target recognition. J Biol Chem. 2011 Jun 3;286(22):19652-61. Epub 2011 Apr 7. PMID:21474448 doi:10.1074/jbc.M110.200428
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