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| | ==HIV-1 neutralizing antibody Z13e1 in complex with epitope display protein== | | ==HIV-1 neutralizing antibody Z13e1 in complex with epitope display protein== |
| - | <StructureSection load='3q1s' size='340' side='right' caption='[[3q1s]], [[Resolution|resolution]] 2.15Å' scene=''> | + | <StructureSection load='3q1s' size='340' side='right'caption='[[3q1s]], [[Resolution|resolution]] 2.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3q1s]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q1S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q1S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3q1s]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q1S FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3fn0|3fn0]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IL22, ILTIF, ZCYTO18, UNQ3099/PRO10096 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q1s OCA], [https://pdbe.org/3q1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q1s RCSB], [https://www.ebi.ac.uk/pdbsum/3q1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q1s ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q1s OCA], [http://pdbe.org/3q1s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3q1s RCSB], [http://www.ebi.ac.uk/pdbsum/3q1s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3q1s ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/IL22_HUMAN IL22_HUMAN]] Cytokine that contributes to the inflammatory response in vivo. | + | [https://www.uniprot.org/uniprot/IL22_HUMAN IL22_HUMAN] Cytokine that contributes to the inflammatory response in vivo. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Interleukin|Interleukin]] | + | *[[Interleukin 3D structures|Interleukin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Gach, J S]] | + | [[Category: Large Structures]] |
| - | [[Category: Julien, J P]] | + | [[Category: Gach JS]] |
| - | [[Category: Pejchal, R]] | + | [[Category: Julien J-P]] |
| - | [[Category: Stanfield, R L]] | + | [[Category: Pejchal R]] |
| - | [[Category: Wilson, I A]] | + | [[Category: Stanfield RL]] |
| - | [[Category: Zwick, M B]] | + | [[Category: Wilson IA]] |
| - | [[Category: Antibody]]
| + | [[Category: Zwick MB]] |
| - | [[Category: Gp41]]
| + | |
| - | [[Category: Immune system-cytokine complex]]
| + | |
| - | [[Category: Immunoglobulin fold]]
| + | |
| Structural highlights
Function
IL22_HUMAN Cytokine that contributes to the inflammatory response in vivo.
Publication Abstract from PubMed
Antibody Z13e1 is a relatively broadly neutralizing anti-human immunodeficiency virus type 1 antibody that recognizes the membrane-proximal external region (MPER) of the human immunodeficiency virus type 1 envelope glycoprotein gp41. Based on the crystal structure of an MPER epitope peptide in complex with Z13e1 Fab, we identified an unrelated protein, interleukin (IL)-22, with a surface-exposed region that is structurally homologous in its backbone to the gp41 Z13e1 epitope. By grafting the gp41 Z13e1 epitope sequence onto the structurally homologous region in IL-22, we engineered a novel protein (Z13-IL22-2) that contains the MPER epitope sequence for use as a potential immunogen and as a reagent for the detection of Z13e1-like antibodies. The Z13-IL22-2 protein binds Fab Z13e1 with a K(d) of 73 nM. The crystal structure of Z13-IL22-2 in complex with Fab Z13e1 shows that the epitope region is faithfully replicated in the Fab-bound scaffold protein; however, isothermal calorimetry studies indicate that Fab binding to Z13-IL22-2 is not a lock-and-key event, leaving open the question of whether conformational changes upon binding occur in the Fab, in Z13-IL-22, or in both.
Structure-Based Design of a Protein Immunogen that Displays an HIV-1 gp41 Neutralizing Epitope.,Stanfield RL, Julien JP, Pejchal R, Gach JS, Zwick MB, Wilson IA J Mol Biol. 2011 Dec 2;414(3):460-76. Epub 2011 Oct 15. PMID:22033480[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Stanfield RL, Julien JP, Pejchal R, Gach JS, Zwick MB, Wilson IA. Structure-Based Design of a Protein Immunogen that Displays an HIV-1 gp41 Neutralizing Epitope. J Mol Biol. 2011 Dec 2;414(3):460-76. Epub 2011 Oct 15. PMID:22033480 doi:10.1016/j.jmb.2011.10.014
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