1ma8

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[[Image:1ma8.gif|left|200px]]
 
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{{Structure
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==A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine==
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|PDB= 1ma8 |SIZE=350|CAPTION= <scene name='initialview01'>1ma8</scene>, resolution 1.3&Aring;
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<StructureSection load='1ma8' size='340' side='right'caption='[[1ma8]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=UMS:2&#39;-METHYLSELENYL-2&#39;-DEOXYURIDINE-5&#39;-PHOSPHATE'>UMS</scene>
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<table><tr><td colspan='2'>[[1ma8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MA8 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ma8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ma8 OCA], [https://pdbe.org/1ma8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ma8 RCSB], [https://www.ebi.ac.uk/pdbsum/1ma8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ma8 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ma8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ma8 OCA], [http://www.ebi.ac.uk/pdbsum/1ma8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ma8 RCSB]</span>
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__TOC__
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}}
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</StructureSection>
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[[Category: Large Structures]]
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'''A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine'''
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[[Category: Carrasco N]]
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[[Category: Du Q]]
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[[Category: Egli M]]
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==Overview==
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[[Category: Huang Z]]
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Selenium was incorporated into an oligodeoxynucleotide in the form of 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 A, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2'-alpha-position with selenium does not affect the preference of the sugar for the C3'-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines.
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[[Category: Teplova M]]
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[[Category: Tereshko V]]
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==About this Structure==
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[[Category: Wawrzak Z]]
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1MA8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA].
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[[Category: Wilds CJ]]
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==Reference==
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Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle. Multiwavelength anomalous dispersion., Teplova M, Wilds CJ, Wawrzak Z, Tereshko V, Du Q, Carrasco N, Huang Z, Egli M, Biochimie. 2002 Sep;84(9):849-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12458077 12458077]
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[[Category: Protein complex]]
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[[Category: Carrasco, N.]]
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[[Category: Du, Q.]]
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[[Category: Egli, M.]]
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[[Category: Huang, Z.]]
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[[Category: Teplova, M.]]
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[[Category: Tereshko, V.]]
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[[Category: Wawrzak, Z.]]
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[[Category: Wilds, C J.]]
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[[Category: 2'-methylseleno-uridine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:13:26 2008''
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Current revision

A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine

PDB ID 1ma8

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