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| | ==Crystal structure of Escherichia coli PliG in complex with Atlantic salmon g-type lysozyme== | | ==Crystal structure of Escherichia coli PliG in complex with Atlantic salmon g-type lysozyme== |
| - | <StructureSection load='4g9s' size='340' side='right' caption='[[4g9s]], [[Resolution|resolution]] 0.95Å' scene=''> | + | <StructureSection load='4g9s' size='340' side='right'caption='[[4g9s]], [[Resolution|resolution]] 0.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4g9s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Atlantic_salmon Atlantic salmon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G9S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4g9s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Salmo_salar Salmo salar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G9S FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.95Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lysG, LYG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8030 Atlantic salmon]), pliG, ycgK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g9s OCA], [http://pdbe.org/4g9s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g9s RCSB], [http://www.ebi.ac.uk/pdbsum/4g9s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g9s ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g9s OCA], [https://pdbe.org/4g9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g9s RCSB], [https://www.ebi.ac.uk/pdbsum/4g9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g9s ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PLIG_ECOLI PLIG_ECOLI]] Inhibits activity of g-type lysozyme, which confers increased lysozyme tolerance to the bacterium. | + | [https://www.uniprot.org/uniprot/A6PZ97_SALSA A6PZ97_SALSA] |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Gram-negative bacteria can produce specific proteinaceous inhibitors to defend themselves against the lytic action of host lysozymes. So far, four different lysozyme inhibitor families have been identified. Here, we report the crystal structure of the Escherichia coli periplasmic lysozyme inhibitor of g-type lysozyme (PliG-Ec) in complex with Atlantic salmon g-type lysozyme (SalG) at a resolution of 0.95 A, which is exceptionally high for a complex of two proteins. The structure reveals for the first time the mechanism of g-type lysozyme inhibition by the PliG family. The latter contains two specific conserved regions that are essential for its inhibitory activity. The inhibitory complex formation is based on a double 'key-lock' mechanism. The first key-lock element is formed by the insertion of two conserved PliG regions into the active site of the lysozyme. The second element is defined by a distinct pocket of PliG accommodating a lysozyme loop. Computational analysis indicates that this pocket represents a suitable site for small molecule binding, which opens an avenue for the development of novel antibacterial agents that suppress the inhibitory activity of PliG.
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| - | Structural basis of bacterial defense against g-type lysozyme-based innate immunity.,Leysen S, Vanderkelen L, Weeks SD, Michiels CW, Strelkov SV Cell Mol Life Sci. 2012 Oct 21. PMID:23086131<ref>PMID:23086131</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 4g9s" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
| | *[[Lysozyme 3D structures|Lysozyme 3D structures]] | | *[[Lysozyme 3D structures|Lysozyme 3D structures]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| - | [[Category: Atlantic salmon]] | + | [[Category: Large Structures]] |
| - | [[Category: Leysen, S]] | + | [[Category: Salmo salar]] |
| - | [[Category: Michiels, C W]] | + | [[Category: Leysen S]] |
| - | [[Category: Strelkov, S V]]
| + | [[Category: Michiels CW]] |
| - | [[Category: Vanderkelen, L]] | + | [[Category: Strelkov SV]] |
| - | [[Category: Weeks, S D]] | + | [[Category: Vanderkelen L]] |
| - | [[Category: Hydrolase inhibitor]] | + | [[Category: Weeks SD]] |
| - | [[Category: Hydrolase-hydrolase inhibitor complex]] | + | |
| - | [[Category: Lysozyme]]
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