3j0r

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==Model of a type III secretion system needle based on a 7 Angstrom resolution cryoEM map==
==Model of a type III secretion system needle based on a 7 Angstrom resolution cryoEM map==
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<StructureSection load='3j0r' size='340' side='right' caption='[[3j0r]], [[Resolution|resolution]] 7.70&Aring;' scene=''>
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<SX load='3j0r' size='340' side='right' viewer='molstar' caption='[[3j0r]], [[Resolution|resolution]] 7.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3j0r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J0R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J0R FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3j0r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J0R FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j0r OCA], [http://pdbe.org/3j0r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j0r RCSB], [http://www.ebi.ac.uk/pdbsum/3j0r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j0r ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j0r OCA], [https://pdbe.org/3j0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j0r RCSB], [https://www.ebi.ac.uk/pdbsum/3j0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j0r ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/MXIH_SHIFL MXIH_SHIFL]] Necessary for the secretion of IPA invasins.
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[https://www.uniprot.org/uniprot/MXIH_SHIFL MXIH_SHIFL] Necessary for the secretion of IPA invasins.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Type III secretion systems of Gram-negative bacteria form injection devices that deliver effector proteins into eukaryotic cells during infection. They span both bacterial membranes and the extracellular space to connect with the host cell plasma membrane. Their extracellular portion is a needle-like, hollow tube that serves as a secretion conduit for effector proteins. The needle of Shigella flexneri is approximately 50-nm long and 7-nm thick and is made by the helical assembly of one protein, MxiH. We provide a 7-A resolution 3D image reconstruction of the Shigella needle by electron cryomicroscopy, which resolves alpha-helices and a beta-hairpin that has never been observed in the crystal and solution structures of needle proteins, including MxiH. An atomic model of the needle based on the 3D-density map, in comparison with that of the bacterial-flagellar filament, provides insights into how such a thin tubular structure is stably assembled by intricate intermolecular interactions. The map also illuminates how the needle-length control protein functions as a ruler within the central channel during export of MxiH for assembly at the distal end of the needle, and how the secretion-activation signal may be transduced through a conformational change of the needle upon host-cell contact.
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Structure of a type III secretion needle at 7-A resolution provides insights into its assembly and signaling mechanisms.,Fujii T, Cheung M, Blanco A, Kato T, Blocker AJ, Namba K Proc Natl Acad Sci U S A. 2012 Mar 2. PMID:22388746<ref>PMID:22388746</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3j0r" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: Large Structures]]
[[Category: Shigella flexneri]]
[[Category: Shigella flexneri]]
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[[Category: Blanco, A]]
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[[Category: Blanco A]]
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[[Category: Blocker, A J]]
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[[Category: Blocker AJ]]
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[[Category: Cheung, M]]
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[[Category: Cheung M]]
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[[Category: Fujii, T]]
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[[Category: Fujii T]]
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[[Category: Kato, T]]
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[[Category: Kato T]]
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[[Category: Namba, K]]
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[[Category: Namba K]]
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[[Category: Helical assembly]]
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[[Category: Protein transport]]
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Current revision

Model of a type III secretion system needle based on a 7 Angstrom resolution cryoEM map

3j0r, resolution 7.70Å

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