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1mc1

From Proteopedia

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[[Image:1mc1.gif|left|200px]]
 
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{{Structure
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==BETA-LACTAM SYNTHETASE WITH PRODUCT (DGPC), AMP AND PPI==
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|PDB= 1mc1 |SIZE=350|CAPTION= <scene name='initialview01'>1mc1</scene>, resolution 2.16&Aring;
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<StructureSection load='1mc1' size='340' side='right'caption='[[1mc1]], [[Resolution|resolution]] 2.16&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PCX:DEOXYGUANIDINOPROCLAVAMINIC+ACID'>PCX</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>
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<table><tr><td colspan='2'>[[1mc1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MC1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MC1 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.16&#8491;</td></tr>
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|GENE= 1901 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 Streptomyces clavuligerus])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PCX:DEOXYGUANIDINOPROCLAVAMINIC+ACID'>PCX</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mc1 OCA], [https://pdbe.org/1mc1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mc1 RCSB], [https://www.ebi.ac.uk/pdbsum/1mc1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mc1 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1jgt|1JGT]], [[1m1z|1M1Z]], [[1mb9|1MB9]], [[1mbz|1MBZ]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mc1 OCA], [http://www.ebi.ac.uk/pdbsum/1mc1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mc1 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/BLS_STRCL BLS_STRCL]
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== Evolutionary Conservation ==
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'''BETA-LACTAM SYNTHETASE WITH PRODUCT (DGPC), AMP AND PPI'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mc/1mc1_consurf.spt"</scriptWhenChecked>
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The catalytic cycle of the ATP/Mg(2+)-dependent enzyme beta-lactam synthetase (beta-LS) from Streptomyces clavuligerus has been observed through a series of x-ray crystallographic snapshots. Chemistry is initiated by the ordered binding of ATP/Mg(2+) and N(2)-(carboxyethyl)-l-arginine (CEA) to the apoenzyme. The apo and ATP/Mg(2+) structures described here, along with the previously described CEA.alpha,beta-methyleneadenosine 5'-triphosphate (CEA.AMP-CPP)/Mg(2+) structure, illuminate changes in active site geometry that favor adenylation. In addition, an acyladenylate intermediate has been trapped. The substrate analog N(2)-(carboxymethyl)-l-arginine (CMA) was adenylated by ATP in the crystal and represents a close structural analog of the previously proposed CEA-adenylate intermediate. Finally, the structure of the ternary product complex deoxyguanidinoproclavaminic acid (DGPC).AMP/PP(i)/Mg(2+) has been determined. The CMA-AMP/PP(i)/Mg(2+) and DGPC.AMP/PP(i)/Mg(2+) structures reveal interactions in the active site that facilitate beta-lactam formation. All of the ATP-bound structures differ from the previously described CEA.AMP-CPP/Mg(2+) structure in that two Mg(2+) ions are found in the active sites. These Mg(2+) ions play critical roles in both the adenylation and beta-lactamization reactions.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1MC1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MC1 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mc1 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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The catalytic cycle of beta -lactam synthetase observed by x-ray crystallographic snapshots., Miller MT, Bachmann BO, Townsend CA, Rosenzweig AC, Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14752-7. Epub 2002 Oct 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12409610 12409610]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Large Structures]]
[[Category: Streptomyces clavuligerus]]
[[Category: Streptomyces clavuligerus]]
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[[Category: Bachmann, B O.]]
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[[Category: Bachmann BO]]
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[[Category: Miller, M T.]]
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[[Category: Miller MT]]
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[[Category: Rosenzweig, A C.]]
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[[Category: Rosenzweig AC]]
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[[Category: Townsend, C A.]]
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[[Category: Townsend CA]]
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[[Category: asparagine synthetase]]
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[[Category: beta-lactam synthetase]]
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[[Category: carboxyethyl arginine]]
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[[Category: clavulanic acid]]
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[[Category: deoxyguanidinoproclavaminic acid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:14:11 2008''
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Current revision

BETA-LACTAM SYNTHETASE WITH PRODUCT (DGPC), AMP AND PPI

PDB ID 1mc1

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