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| | ==CANINE ADENOVIRUS TYPE 2 FIBRE HEAD IN COMPLEX WITH CAR DOMAIN D1 AND SIALIC ACID== | | ==CANINE ADENOVIRUS TYPE 2 FIBRE HEAD IN COMPLEX WITH CAR DOMAIN D1 AND SIALIC ACID== |
| - | <StructureSection load='2w9l' size='340' side='right' caption='[[2w9l]], [[Resolution|resolution]] 2.91Å' scene=''> | + | <StructureSection load='2w9l' size='340' side='right'caption='[[2w9l]], [[Resolution|resolution]] 2.91Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2w9l]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Adec2 Adec2] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W9L FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2w9l]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Canine_adenovirus_2 Canine adenovirus 2] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W9L FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GL0:BETA-D-GULOPYRANOSE'>GL0</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.91Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GL0:BETA-D-GULOPYRANOSE'>GL0</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2j12|2j12]], [[1eaj|1eaj]], [[1p69|1p69]], [[1jew|1jew]], [[1kac|1kac]], [[2j2j|2j2j]], [[1p6a|1p6a]], [[1rsf|1rsf]], [[1f5w|1f5w]], [[2j1k|2j1k]]</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9l OCA], [https://pdbe.org/2w9l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w9l RCSB], [https://www.ebi.ac.uk/pdbsum/2w9l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w9l ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9l OCA], [http://pdbe.org/2w9l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w9l RCSB], [http://www.ebi.ac.uk/pdbsum/2w9l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w9l ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CXAR_HUMAN CXAR_HUMAN]] Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN.<ref>PMID:9096397</ref> <ref>PMID:11734628</ref> <ref>PMID:12297051</ref> <ref>PMID:15800062</ref> | + | [https://www.uniprot.org/uniprot/CXAR_HUMAN CXAR_HUMAN] Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN.<ref>PMID:9096397</ref> <ref>PMID:11734628</ref> <ref>PMID:12297051</ref> <ref>PMID:15800062</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w9/2w9l_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w9/2w9l_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Adec2]] | + | [[Category: Canine adenovirus 2]] |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Billet, O]] | + | [[Category: Large Structures]] |
| - | [[Category: Cusack, S]] | + | [[Category: Billet O]] |
| - | [[Category: Dreja, H]] | + | [[Category: Cusack S]] |
| - | [[Category: Finberg, R W]] | + | [[Category: Dreja H]] |
| - | [[Category: Henaff, D]] | + | [[Category: Finberg RW]] |
| - | [[Category: Hippert, C]] | + | [[Category: Henaff D]] |
| - | [[Category: Ibanes, S]] | + | [[Category: Hippert C]] |
| - | [[Category: Kalatzis, V]] | + | [[Category: Ibanes S]] |
| - | [[Category: Kremer, E J]] | + | [[Category: Kalatzis V]] |
| - | [[Category: Lortat-Jacob, H]] | + | [[Category: Kremer EJ]] |
| - | [[Category: Mennechet, F]] | + | [[Category: Lortat-Jacob H]] |
| - | [[Category: Perreau, M]] | + | [[Category: Mennechet F]] |
| - | [[Category: Schoehn, G]] | + | [[Category: Perreau M]] |
| - | [[Category: Seiradake, E]] | + | [[Category: Schoehn G]] |
| - | [[Category: Wang, J P]] | + | [[Category: Seiradake E]] |
| - | [[Category: Wodrich, H]] | + | [[Category: Wang JP]] |
| - | [[Category: Adenovirus]]
| + | [[Category: Wodrich H]] |
| - | [[Category: Canine]]
| + | |
| - | [[Category: Car]]
| + | |
| - | [[Category: Cav-2]]
| + | |
| - | [[Category: Cell adhesion]]
| + | |
| - | [[Category: Cell junction]]
| + | |
| - | [[Category: Cell membrane]]
| + | |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Coxsackievirus]]
| + | |
| - | [[Category: Domain d1]]
| + | |
| - | [[Category: Erythrocyte]]
| + | |
| - | [[Category: Fiber]]
| + | |
| - | [[Category: Fiber head]]
| + | |
| - | [[Category: Fiber protein]]
| + | |
| - | [[Category: Fibre]]
| + | |
| - | [[Category: Fibre head]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Hemagglutination]]
| + | |
| - | [[Category: Host-virus interaction]]
| + | |
| - | [[Category: Immunoglobulin domain]]
| + | |
| - | [[Category: Knob]]
| + | |
| - | [[Category: Lipoprotein]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Palmitate]]
| + | |
| - | [[Category: Phosphoprotein]]
| + | |
| - | [[Category: Phosphorylation]]
| + | |
| - | [[Category: Receptor]]
| + | |
| - | [[Category: Red blood cell]]
| + | |
| - | [[Category: Secreted]]
| + | |
| - | [[Category: Sialic acid]]
| + | |
| - | [[Category: Sialyl-lactose]]
| + | |
| - | [[Category: Tight junction]]
| + | |
| - | [[Category: Transmembrane]]
| + | |
| - | [[Category: Virus-receptor complex]]
| + | |
| Structural highlights
Function
CXAR_HUMAN Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN.[1] [2] [3] [4]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Although it has been known for 50 years that adenoviruses (Ads) interact with erythrocytes ex vivo, the molecular and structural basis for this interaction, which has been serendipitously exploited for diagnostic tests, is unknown. In this study, we characterized the interaction between erythrocytes and unrelated Ad serotypes, human 5 (HAd5) and 37 (HAd37), and canine 2 (CAV-2). While these serotypes agglutinate human erythrocytes, they use different receptors, have different tropisms and/or infect different species. Using molecular, biochemical, structural and transgenic animal-based analyses, we found that the primary erythrocyte interaction domain for HAd37 is its sialic acid binding site, while CAV-2 binding depends on at least three factors: electrostatic interactions, sialic acid binding and, unexpectedly, binding to the coxsackievirus and adenovirus receptor (CAR) on human erythrocytes. We show that the presence of CAR on erythrocytes leads to prolonged in vivo blood half-life and significantly reduced liver infection when a CAR-tropic Ad is injected intravenously. This study provides i) a molecular and structural rationale for Ad-erythrocyte interactions, ii) a basis to improve vector-mediated gene transfer and iii) a mechanism that may explain the biodistribution and pathogenic inconsistencies found between human and animal models.
The cell adhesion molecule "CAR" and sialic acid on human erythrocytes influence adenovirus in vivo biodistribution.,Seiradake E, Henaff D, Wodrich H, Billet O, Perreau M, Hippert C, Mennechet F, Schoehn G, Lortat-Jacob H, Dreja H, Ibanes S, Kalatzis V, Wang JP, Finberg RW, Cusack S, Kremer EJ PLoS Pathog. 2009 Jan;5(1):e1000277. Epub 2009 Jan 2. PMID:19119424[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Tomko RP, Xu R, Philipson L. HCAR and MCAR: the human and mouse cellular receptors for subgroup C adenoviruses and group B coxsackieviruses. Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3352-6. PMID:9096397
- ↑ Cohen CJ, Shieh JT, Pickles RJ, Okegawa T, Hsieh JT, Bergelson JM. The coxsackievirus and adenovirus receptor is a transmembrane component of the tight junction. Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15191-6. Epub 2001 Dec 4. PMID:11734628 doi:10.1073/pnas.261452898
- ↑ Walters RW, Freimuth P, Moninger TO, Ganske I, Zabner J, Welsh MJ. Adenovirus fiber disrupts CAR-mediated intercellular adhesion allowing virus escape. Cell. 2002 Sep 20;110(6):789-99. PMID:12297051
- ↑ Zen K, Liu Y, McCall IC, Wu T, Lee W, Babbin BA, Nusrat A, Parkos CA. Neutrophil migration across tight junctions is mediated by adhesive interactions between epithelial coxsackie and adenovirus receptor and a junctional adhesion molecule-like protein on neutrophils. Mol Biol Cell. 2005 Jun;16(6):2694-703. Epub 2005 Mar 30. PMID:15800062 doi:E05-01-0036
- ↑ Seiradake E, Henaff D, Wodrich H, Billet O, Perreau M, Hippert C, Mennechet F, Schoehn G, Lortat-Jacob H, Dreja H, Ibanes S, Kalatzis V, Wang JP, Finberg RW, Cusack S, Kremer EJ. The cell adhesion molecule "CAR" and sialic acid on human erythrocytes influence adenovirus in vivo biodistribution. PLoS Pathog. 2009 Jan;5(1):e1000277. Epub 2009 Jan 2. PMID:19119424 doi:10.1371/journal.ppat.1000277
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