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3zl9

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==Crystal structure of the nucleocapsid protein from Schmallenberg virus==
==Crystal structure of the nucleocapsid protein from Schmallenberg virus==
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<StructureSection load='3zl9' size='340' side='right' caption='[[3zl9]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
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<StructureSection load='3zl9' size='340' side='right'caption='[[3zl9]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3zl9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sbv Sbv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZL9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZL9 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3zl9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Schmallenberg_virus Schmallenberg virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZL9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZL9 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zla|3zla]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zl9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zl9 OCA], [http://pdbe.org/3zl9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zl9 RCSB], [http://www.ebi.ac.uk/pdbsum/3zl9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zl9 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zl9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zl9 OCA], [https://pdbe.org/3zl9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zl9 RCSB], [https://www.ebi.ac.uk/pdbsum/3zl9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zl9 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/NCAP_SBVBH NCAP_SBVBH] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC) (By similarity).
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All orthobunyaviruses possess three genome segments of single-stranded negative sense RNA that are encapsidated with the virus-encoded nucleocapsid (N) protein to form a ribonucleoprotein (RNP) complex, which is uncharacterized at high resolution. We report the crystal structure of both the Bunyamwera virus (BUNV) N-RNA complex and the unbound Schmallenberg virus (SBV) N protein, at resolutions of 3.20 and 2.75 A, respectively. Both N proteins crystallized as ring-like tetramers and exhibit a high degree of structural similarity despite classification into different orthobunyavirus serogroups. The structures represent a new RNA-binding protein fold. BUNV N possesses a positively charged groove into which RNA is deeply sequestered, with the bases facing away from the solvent. This location is highly inaccessible, implying that RNA polymerization and other critical base pairing events in the virus life cycle require RNP disassembly. Mutational analysis of N protein supports a correlation between structure and function. Comparison between these crystal structures and electron microscopy images of both soluble tetramers and authentic RNPs suggests the N protein does not bind RNA as a repeating monomer; thus, it represents a newly described architecture for bunyavirus RNP assembly, with implications for many other segmented negative-strand RNA viruses.
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Nucleocapsid protein structures from orthobunyaviruses reveal insight into ribonucleoprotein architecture and RNA polymerization.,Ariza A, Tanner SJ, Walter CT, Dent KC, Shepherd DA, Wu W, Matthews SV, Hiscox JA, Green TJ, Luo M, Elliott RM, Fooks AR, Ashcroft AE, Stonehouse NJ, Ranson NA, Barr JN, Edwards TA Nucleic Acids Res. 2013 Apr 17. PMID:23595147<ref>PMID:23595147</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3zl9" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Sbv]]
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[[Category: Large Structures]]
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[[Category: Ariza, A]]
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[[Category: Schmallenberg virus]]
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[[Category: Ashcroft, A E]]
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[[Category: Ariza A]]
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[[Category: Barr, J N]]
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[[Category: Ashcroft AE]]
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[[Category: Dent, K C]]
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[[Category: Barr JN]]
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[[Category: Edwards, T A]]
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[[Category: Dent KC]]
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[[Category: Elliot, R M]]
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[[Category: Edwards TA]]
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[[Category: Green, T J]]
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[[Category: Elliot RM]]
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[[Category: Hiscox, J A]]
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[[Category: Green TJ]]
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[[Category: Luo, M]]
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[[Category: Hiscox JA]]
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[[Category: Matthews, S V]]
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[[Category: Luo M]]
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[[Category: Ranson, N A]]
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[[Category: Matthews SV]]
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[[Category: Shepherd, D A]]
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[[Category: Ranson NA]]
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[[Category: Stonehouse, N J]]
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[[Category: Shepherd DA]]
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[[Category: Tanner, S J]]
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[[Category: Stonehouse NJ]]
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[[Category: Walter, C T]]
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[[Category: Tanner SJ]]
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[[Category: Wu, W]]
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[[Category: Walter CT]]
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[[Category: Nucleoprotein]]
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[[Category: Wu W]]
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[[Category: Orthobunyavirus]]
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[[Category: Viral protein]]
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Current revision

Crystal structure of the nucleocapsid protein from Schmallenberg virus

PDB ID 3zl9

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