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| | ==Crystal Structures of MthK RCK gating ring bound to Barium== | | ==Crystal Structures of MthK RCK gating ring bound to Barium== |
| - | <StructureSection load='4ei2' size='340' side='right' caption='[[4ei2]], [[Resolution|resolution]] 3.11Å' scene=''> | + | <StructureSection load='4ei2' size='340' side='right'caption='[[4ei2]], [[Resolution|resolution]] 3.11Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ei2]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Metth Metth]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EI2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EI2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ei2]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus_str._Delta_H Methanothermobacter thermautotrophicus str. Delta H]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EI2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EI2 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.108Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rbz|3rbz]], [[2fy8|2fy8]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mthK, MTH_1520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=187420 METTH])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ei2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ei2 OCA], [https://pdbe.org/4ei2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ei2 RCSB], [https://www.ebi.ac.uk/pdbsum/4ei2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ei2 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ei2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ei2 OCA], [http://pdbe.org/4ei2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ei2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ei2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ei2 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH]] Calcium-gated potassium channel. | + | [https://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH] Calcium-gated potassium channel. |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | RCK domains control activity of a variety of K(+) channels and transporters through binding of cytoplasmic ligands. To gain insight toward mechanisms of RCK domain activation, we solved the structure of the RCK domain from the Ca(2+)-gated K(+) channel, MthK, bound with Ba(2+), at 3.1 A resolution. The Ba(2+)-bound RCK domain was assembled as an octameric gating ring, as observed in structures of the full-length MthK channel, and shows Ba(2+) bound at several positions. One of the Ba(2+) sites, termed C1, overlaps with a known Ca(2+)-activation site, determined by residues D184 and E210. Functionally, Ba(2+) can activate reconstituted MthK channels as observed in electrophysiological recordings, whereas Mg(2+) (up to 100 mM) was ineffective. Ba(2+) activation was abolished by the mutation D184N, suggesting that Ba(2+) activates primarily through the C1 site. Our results suggest a working hypothesis for a sequence of ligand-dependent conformational changes that may underlie RCK domain activation and channel gating.
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| - | Crystal Structure of a Ba(2+)-Bound Gating Ring Reveals Elementary Steps in RCK Domain Activation.,Smith FJ, Pau VP, Cingolani G, Rothberg BS Structure. 2012 Dec 5;20(12):2038-47. doi: 10.1016/j.str.2012.09.014. Epub 2012, Oct 18. PMID:23085076<ref>PMID:23085076</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 4ei2" style="background-color:#fffaf0;"></div>
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| | ==See Also== | | ==See Also== |
| - | *[[Potassium Channel|Potassium Channel]] | + | *[[Potassium channel 3D structures|Potassium channel 3D structures]] |
| - | == References ==
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| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Metth]] | + | [[Category: Large Structures]] |
| - | [[Category: Cingolani, G]] | + | [[Category: Methanothermobacter thermautotrophicus str. Delta H]] |
| - | [[Category: Rothberg, B S]] | + | [[Category: Cingolani G]] |
| - | [[Category: Smith, F J]] | + | [[Category: Rothberg BS]] |
| - | [[Category: Ba2+ binding]] | + | [[Category: Smith FJ]] |
| - | [[Category: K+ channel]]
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| - | [[Category: Membrane protein]]
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| - | [[Category: Rck domain]]
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| - | [[Category: Rossmann-fold]]
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| - | [[Category: Transport protein]]
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