4kf5

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==Crystal Structure of Split GFP complexed with engineered sfCherry with an insertion of GFP fragment==
==Crystal Structure of Split GFP complexed with engineered sfCherry with an insertion of GFP fragment==
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<StructureSection load='4kf5' size='340' side='right' caption='[[4kf5]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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<StructureSection load='4kf5' size='340' side='right'caption='[[4kf5]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4kf5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KF5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KF5 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4kf5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KF5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KF5 FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CH6:{(4Z)-2-[(1S)-1-AMINO-3-(METHYLSULFANYL)PROPYL]-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CH6</scene>, <scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.599&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4kf4|4kf4]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CH6:{(4Z)-2-[(1S)-1-AMINO-3-(METHYLSULFANYL)PROPYL]-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CH6</scene>, <scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kf5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kf5 OCA], [http://pdbe.org/4kf5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kf5 RCSB], [http://www.ebi.ac.uk/pdbsum/4kf5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kf5 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kf5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kf5 OCA], [https://pdbe.org/4kf5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kf5 RCSB], [https://www.ebi.ac.uk/pdbsum/4kf5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kf5 ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Synthetic construct sequences]]
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[[Category: Large Structures]]
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[[Category: Hung, L W]]
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[[Category: Synthetic construct]]
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[[Category: Nguyen, H B]]
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[[Category: Hung L-W]]
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[[Category: Terwilliger, T C]]
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[[Category: Nguyen HB]]
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[[Category: Waldo, G S]]
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[[Category: Terwilliger TC]]
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[[Category: Yeates, T O]]
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[[Category: Waldo GS]]
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[[Category: Fluorescent protein]]
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[[Category: Yeates TO]]

Current revision

Crystal Structure of Split GFP complexed with engineered sfCherry with an insertion of GFP fragment

PDB ID 4kf5

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