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| | ==T. thermophilus RuvC in complex with Holliday junction substrate== | | ==T. thermophilus RuvC in complex with Holliday junction substrate== |
| - | <StructureSection load='4ld0' size='340' side='right' caption='[[4ld0]], [[Resolution|resolution]] 3.75Å' scene=''> | + | <StructureSection load='4ld0' size='340' side='right'caption='[[4ld0]], [[Resolution|resolution]] 3.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ld0]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LD0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LD0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ld0]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LD0 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ruvC, TTHA1090 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.75Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Crossover_junction_endodeoxyribonuclease Crossover junction endodeoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.22.4 3.1.22.4] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ld0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ld0 OCA], [https://pdbe.org/4ld0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ld0 RCSB], [https://www.ebi.ac.uk/pdbsum/4ld0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ld0 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ld0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ld0 OCA], [http://pdbe.org/4ld0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ld0 RCSB], [http://www.ebi.ac.uk/pdbsum/4ld0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ld0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RUVC_THET8 RUVC_THET8]] Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group (By similarity). | + | [https://www.uniprot.org/uniprot/RUVC_THET8 RUVC_THET8] Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group (By similarity). |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | The key intermediate in genetic recombination is the Holliday junction (HJ), a four-way DNA structure. At the end of recombination, HJs are cleaved by specific nucleases called resolvases. In Gram-negative bacteria, this cleavage is performed by RuvC, a dimeric endonuclease that belongs to the retroviral integrase superfamily. Here, we report the first crystal structure of RuvC in complex with a synthetic HJ solved at 3.75 A resolution. The junction in the complex is in an unfolded 2-fold symmetrical conformation, in which the four arms point toward the vertices of a tetrahedron. The two scissile phosphates are located one nucleotide from the strand exchange point, and RuvC approaches them from the minor groove side. The key protein-DNA contacts observed in the structure were verified using a thiol-based site-specific cross-linking approach. Compared with known complex structures of the phage resolvases endonuclease I and endonuclease VII, the RuvC structure exhibits striking differences in the mode of substrate binding and location of the cleavage site.
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| - | Crystal structure of RuvC resolvase in complex with Holliday junction substrate.,Gorecka KM, Komorowska W, Nowotny M Nucleic Acids Res. 2013 Sep 18. PMID:23980027<ref>PMID:23980027</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 4ld0" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Crossover junction endodeoxyribonuclease]] | + | [[Category: Large Structures]] |
| - | [[Category: Thet8]] | + | [[Category: Thermus thermophilus HB8]] |
| - | [[Category: Gorecka, K M]] | + | [[Category: Gorecka KM]] |
| - | [[Category: Komorowska, W]] | + | [[Category: Komorowska W]] |
| - | [[Category: Nowotny, M]] | + | [[Category: Nowotny M]] |
| - | [[Category: Dna]]
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| - | [[Category: Hydrolase-dna complex]]
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| - | [[Category: Nuclease]]
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| - | [[Category: Rnase h fold]]
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