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| | ==hexameric crystal structure of Schmallenberg virus nucleoprotein== | | ==hexameric crystal structure of Schmallenberg virus nucleoprotein== |
| - | <StructureSection load='4idx' size='340' side='right' caption='[[4idx]], [[Resolution|resolution]] 3.21Å' scene=''> | + | <StructureSection load='4idx' size='340' side='right'caption='[[4idx]], [[Resolution|resolution]] 3.21Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4idx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sbv Sbv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IDX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IDX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4idx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Schmallenberg_virus Schmallenberg virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IDX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IDX FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4idu|4idu]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.21Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1133363 SBV])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4idx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4idx OCA], [https://pdbe.org/4idx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4idx RCSB], [https://www.ebi.ac.uk/pdbsum/4idx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4idx ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4idx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4idx OCA], [http://pdbe.org/4idx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4idx RCSB], [http://www.ebi.ac.uk/pdbsum/4idx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4idx ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/NCAP_SBVBH NCAP_SBVBH] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC) (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Nucleoprotein|Nucleoprotein]] | + | *[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Sbv]] | + | [[Category: Large Structures]] |
| - | [[Category: Dong, C J]] | + | [[Category: Schmallenberg virus]] |
| - | [[Category: Dong, H H]] | + | [[Category: Dong C]] |
| - | [[Category: Dna binding protein]] | + | [[Category: Dong H]] |
| - | [[Category: Nucleoprotein]]
| + | |
| - | [[Category: Protects genomic rna]]
| + | |
| - | [[Category: Rna replication and transcription]]
| + | |
| - | [[Category: Sbv nucleoprotein]]
| + | |
| Structural highlights
Function
NCAP_SBVBH Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC) (By similarity).
Publication Abstract from PubMed
Schmallenberg virus (SBV), a newly emerged orthobunyavirus (family Bunyaviridae), has spread rapidly across Europe, and has caused congenital abnormalities in the offspring of cattle, sheep and goats. Like other orthobunyaviruses, SBV contains a tripartite negative-sense RNA genome that encodes four structural and two nonstructural proteins. The nucleoprotein (N) encapsidates the three viral genomic RNA segments and plays a crucial role in viral RNA transcription and replication. Here we report the crystal structure of bacterially-expressed SBV nucleoprotein to 3.06A resolution. The protomer is composed of two domains (N-terminal and C-terminal domains) with flexible N-terminal and C-terminal arms. The N protein has a novel fold, and forms a central positively charged cleft for genomic RNA binding. The nucleoprotein purified under native conditions forms a tetramer, while the nucleoprotein obtained following denaturation and refolding forms a hexamer. Our structural and functional analyses demonstrate that both N-terminal and C-terminal arms are involved in N-N interaction and oligomerization and play an essential role in viral RNA synthesis, suggesting a novel mechanism for viral RNA encapsidation and transcription.
Structure of Schmallenberg orthobunyavirus nucleoprotein suggests a novel mechanism for genome encapsidation.,Dong H, Li P, Elliott RM, Dong C J Virol. 2013 Mar 6. PMID:23468499[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Dong H, Li P, Elliott RM, Dong C. Structure of Schmallenberg orthobunyavirus nucleoprotein suggests a novel mechanism for genome encapsidation. J Virol. 2013 Mar 6. PMID:23468499 doi:10.1128/JVI.00223-13
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