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3tpf

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Crystal structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168

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 ==Crystal structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168==
-<StructureSection load='3tpf' size='340' side='right' caption='[[3tpf]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+<StructureSection load='3tpf' size='340' side='right'caption='[[3tpf]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3tpf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Camje Camje]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TPF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3tpf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni_NCTC_11168_=_ATCC_700819 Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TPF FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1c9y|1c9y]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpf OCA], [https://pdbe.org/3tpf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tpf RCSB], [https://www.ebi.ac.uk/pdbsum/3tpf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tpf ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">argF, Cj0994c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192222 CAMJE])</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpf OCA], [http://pdbe.org/3tpf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tpf RCSB], [http://www.ebi.ac.uk/pdbsum/3tpf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tpf ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/OTC_CAMJE OTC_CAMJE]] Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline (By similarity).[HAMAP-Rule:MF_01109]
+[https://www.uniprot.org/uniprot/OTCA_CAMJE OTCA_CAMJE] Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase (ArgG) involved in the final step in arginine biosynthesis.<ref>PMID:22949186</ref>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-Anabolic ornithine transcarbamoylase (aOTC) catalyzes the reaction between carbamoyl phosphate (CP) and L-ornithine (ORN) to form L-citrulline and phosphate in the urea cycle and L-arginine biosynthesis. The crystal structure of unliganded aOTC from Campylobacter jejuni (Cje aOTC) was determined at 2.7 A resolution and refined to an R(work) of 20.3% and an R(free) of 24.0%. Cje aOTC is a trimer that forms a head-to-head pseudohexamer in the asymmetric unit. Each monomer is composed of an N-terminal CP-binding domain and a C-terminal ORN-binding domain joined by two interdomain helices. The Cje aOTC structure presents an open conformation of the enzyme with a relatively flexible orientation of the ORN-binding domain respective to the CP-binding domain. The conformation of the B2-H3 loop (residues 68-78), which is involved in binding CP in an adjacent subunit of the trimer, differs from that seen in homologous proteins with CP bound. The loop containing the ORN-binding motif (DxxxSMG, residues 223-230) has a conformation that is different from those observed in unliganded OTC structures from other species, but is similar to those in structures with bound ORN analogs. The major differences in tertiary structure between Cje aOTC and human aOTC are described.
-Structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni at 2.7 A resolution.,Shabalin IG, Porebski PJ, Cooper DR, Grabowski M, Onopriyenko O, Grimshaw S, Savchenko A, Chruszcz M, Minor W Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Sep;68(Pt 9):1018-24. Epub, 2012 Aug 29. PMID:22949186<ref>PMID:22949186</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 3tpf" style="background-color:#fffaf0;"></div>
 ==See Also==
-*[[Ornithine carbamoyltransferase|Ornithine carbamoyltransferase]]
+*[[Ornithine carbamoyltransferase 3D structures|Ornithine carbamoyltransferase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Camje]]
+[[Category: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]]
-[[Category: Ornithine carbamoyltransferase]]
+[[Category: Large Structures]]
-[[Category: Anderson, W F]]
+[[Category: Anderson WF]]
-[[Category: Structural genomic]]
+[[Category: Chruszcz M]]
-[[Category: Chruszcz, M]]
+[[Category: Grabowski M]]
-[[Category: Grabowski, M]]
+[[Category: Grimshaw S]]
-[[Category: Grimshaw, S]]
+[[Category: Minor W]]
-[[Category: Minor, W]]
+[[Category: Onopriyenko O]]
-[[Category: Onopriyenko, O]]
+[[Category: Porebski PJ]]
-[[Category: Porebski, P J]]
+[[Category: Savchenko A]]
-[[Category: Savchenko, A]]
+[[Category: Shabalin IG]]
-[[Category: Shabalin, I G]]
-[[Category: Carbamoyl phosphate binding]]
-[[Category: Csgid]]
-[[Category: L-ornithine binding]]
-[[Category: Rossmann fold]]
-[[Category: Transferase]]

3tpf

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Crystal structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168

Structural highlights

Function

See Also

References

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