3vv1

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==Crystal Structure of Caenorhabditis elegans galectin LEC-6==
==Crystal Structure of Caenorhabditis elegans galectin LEC-6==
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<StructureSection load='3vv1' size='340' side='right' caption='[[3vv1]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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<StructureSection load='3vv1' size='340' side='right'caption='[[3vv1]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3vv1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VV1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VV1 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3vv1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VV1 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lec-6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vv1 OCA], [http://pdbe.org/3vv1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vv1 RCSB], [http://www.ebi.ac.uk/pdbsum/3vv1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vv1 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vv1 OCA], [https://pdbe.org/3vv1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vv1 RCSB], [https://www.ebi.ac.uk/pdbsum/3vv1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vv1 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q9N384_CAEEL Q9N384_CAEEL]
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Galectins are a group of lectins that can bind carbohydrate chains containing beta-galactoside units. LEC-6, a member of galectins of Caenorhabditis elegans, binds fucose-containing saccharides. We solved the crystal structure of LEC-6 in complex with galactose-beta1,4-fucose (Galbeta1-4Fuc) at 1.5 A resolution. The overall structure of the protein and the identities of the amino-acid residues binding to the disaccharide are similar to those of other galectins. However, further structural analysis and multiple sequence alignment between LEC-6 and other galectins indicate that a glutamic acid residue (Glu67) is important for the preferential binding between LEC-6 and the fucose moiety of the Galbeta1-4Fuc unit. Frontal affinity chromatography analysis indicated that the affinities of E67D and E67A mutants for Galbeta1-4Fuc are lower than that of wild-type LEC-6. Furthermore, the affinities of Glu67 mutants for an endogenous oligosaccharide, which contains a Galbeta1-4Fuc unit, are drastically reduced relative to that of the wild-type protein. We conclude that the Glu67 in the oligosaccharide-binding site assists the recognition of the fucose moiety by LEC-6.
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Structural basis of preferential binding of fucose-containing saccharide by the Caenorhabditis elegans galectin LEC-6.,Makyio H, Takeuchi T, Tamura M, Nishiyama K, Takahashi H, Natsugari H, Arata Y, Kasai KI, Yamada Y, Wakatsuki S, Kato R Glycobiology. 2013 Mar 28. PMID:23481096<ref>PMID:23481096</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3vv1" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Caeel]]
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[[Category: Caenorhabditis elegans]]
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[[Category: Arata, Y]]
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[[Category: Large Structures]]
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[[Category: Kasai, K]]
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[[Category: Arata Y]]
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[[Category: Kato, R]]
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[[Category: Kasai K]]
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[[Category: Makyio, H]]
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[[Category: Kato R]]
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[[Category: Natsugari, H]]
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[[Category: Makyio H]]
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[[Category: Nishiyama, K]]
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[[Category: Natsugari H]]
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[[Category: Takahashi, H]]
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[[Category: Nishiyama K]]
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[[Category: Takeuchi, T]]
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[[Category: Takahashi H]]
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[[Category: Tamura, M]]
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[[Category: Takeuchi T]]
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[[Category: Wakatsuki, S]]
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[[Category: Tamura M]]
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[[Category: Yamada, Y]]
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[[Category: Wakatsuki S]]
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[[Category: 4-fucose binding]]
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[[Category: Yamada Y]]
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[[Category: Galactose-1]]
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[[Category: Galectin]]
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[[Category: Sugar binding protein]]
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Current revision

Crystal Structure of Caenorhabditis elegans galectin LEC-6

PDB ID 3vv1

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