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| | ==Crystal structure of VosA velvet domain== | | ==Crystal structure of VosA velvet domain== |
| - | <StructureSection load='4n6q' size='340' side='right' caption='[[4n6q]], [[Resolution|resolution]] 1.79Å' scene=''> | + | <StructureSection load='4n6q' size='340' side='right'caption='[[4n6q]], [[Resolution|resolution]] 1.79Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4n6q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/A._nidulans A. nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N6Q FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4n6q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans Aspergillus nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N6Q FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.79Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4n6r|4n6r]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6q OCA], [http://pdbe.org/4n6q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n6q RCSB], [http://www.ebi.ac.uk/pdbsum/4n6q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n6q ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6q OCA], [https://pdbe.org/4n6q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n6q RCSB], [https://www.ebi.ac.uk/pdbsum/4n6q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n6q ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/VOSA_EMENI VOSA_EMENI] Component of the velB-VosA heterodimeric complex that plays a dual role in activating genes associated with spore maturation and repressing certain development-associated genes (PubMed:17912349, PubMed:21152013, PubMed:23049895, PubMed:24391470). Controls primarily the late process of sporulation including trehalose biogenesis (PubMed:17912349). The complex binds DNA through the DNA-binding domain of vosA that recognizes an 11-nucleotide consensus sequence 5'-CTGGCCGCGGC-3' consisting of two motifs in the promoters of key developmental regulatory genes (PubMed:24391470). The vosA-velB complex binds to the beta-glucan synthase fksA gene promoter in asexual spores for repression (PubMed:25960370).[UniProtKB:A0SP16]<ref>PMID:17912349</ref> <ref>PMID:21152013</ref> <ref>PMID:23049895</ref> <ref>PMID:25960370</ref> |
| - | Morphological development of fungi and their combined production of secondary metabolites are both acting in defence and protection. These processes are mainly coordinated by velvet regulators, which contain a yet functionally and structurally uncharacterized velvet domain. Here we demonstrate that the velvet domain of VosA is a novel DNA-binding motif that specifically recognizes an 11-nucleotide consensus sequence consisting of two motifs in the promoters of key developmental regulatory genes. The crystal structure analysis of the VosA velvet domain revealed an unforeseen structural similarity with the Rel homology domain (RHD) of the mammalian transcription factor NF-kappaB. Based on this structural similarity several conserved amino acid residues present in all velvet domains have been identified and shown to be essential for the DNA binding ability of VosA. The velvet domain is also involved in dimer formation as seen in the solved crystal structures of the VosA homodimer and the VosA-VelB heterodimer. These findings suggest that defence mechanisms of both fungi and animals might be governed by structurally related DNA-binding transcription factors.
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| - | The Velvet Family of Fungal Regulators Contains a DNA-Binding Domain Structurally Similar to NF-kappaB.,Ahmed YL, Gerke J, Park HS, Bayram O, Neumann P, Ni M, Dickmanns A, Kim SC, Yu JH, Braus GH, Ficner R PLoS Biol. 2013 Dec;11(12):e1001750. doi: 10.1371/journal.pbio.1001750. Epub 2013, Dec 31. PMID:24391470<ref>PMID:24391470</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div> | + | |
| - | <div class="pdbe-citations 4n6q" style="background-color:#fffaf0;"></div> | + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: A. nidulans]] | + | [[Category: Aspergillus nidulans]] |
| - | [[Category: Ahmed, Y L]] | + | [[Category: Large Structures]] |
| - | [[Category: Dickmanns, A]] | + | [[Category: Ahmed YL]] |
| - | [[Category: Ficner, R]] | + | [[Category: Dickmanns A]] |
| - | [[Category: Neumann, P]] | + | [[Category: Ficner R]] |
| - | [[Category: Beta-sandwich]]
| + | [[Category: Neumann P]] |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Ig-fold]]
| + | |
| - | [[Category: Nfkb]]
| + | |
| - | [[Category: Transcription factor]]
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| - | [[Category: Velb]]
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| Structural highlights
Function
VOSA_EMENI Component of the velB-VosA heterodimeric complex that plays a dual role in activating genes associated with spore maturation and repressing certain development-associated genes (PubMed:17912349, PubMed:21152013, PubMed:23049895, PubMed:24391470). Controls primarily the late process of sporulation including trehalose biogenesis (PubMed:17912349). The complex binds DNA through the DNA-binding domain of vosA that recognizes an 11-nucleotide consensus sequence 5'-CTGGCCGCGGC-3' consisting of two motifs in the promoters of key developmental regulatory genes (PubMed:24391470). The vosA-velB complex binds to the beta-glucan synthase fksA gene promoter in asexual spores for repression (PubMed:25960370).[UniProtKB:A0SP16][1] [2] [3] [4]
References
- ↑ Ni M, Yu JH. A novel regulator couples sporogenesis and trehalose biogenesis in Aspergillus nidulans. PLoS One. 2007 Oct 3;2(10):e970. doi: 10.1371/journal.pone.0000970. PMID:17912349 doi:http://dx.doi.org/10.1371/journal.pone.0000970
- ↑ Sarikaya Bayram O, Bayram O, Valerius O, Park HS, Irniger S, Gerke J, Ni M, Han KH, Yu JH, Braus GH. LaeA control of velvet family regulatory proteins for light-dependent development and fungal cell-type specificity. PLoS Genet. 2010 Dec 2;6(12):e1001226. doi: 10.1371/journal.pgen.1001226. PMID:21152013 doi:http://dx.doi.org/10.1371/journal.pgen.1001226
- ↑ Park HS, Ni M, Jeong KC, Kim YH, Yu JH. The role, interaction and regulation of the velvet regulator VelB in Aspergillus nidulans. PLoS One. 2012;7(9):e45935. doi: 10.1371/journal.pone.0045935. Epub 2012 Sep 25. PMID:23049895 doi:http://dx.doi.org/10.1371/journal.pone.0045935
- ↑ Park HS, Man Yu Y, Lee MK, Jae Maeng P, Chang Kim S, Yu JH. Velvet-mediated repression of beta-glucan synthesis in Aspergillus nidulans spores. Sci Rep. 2015 May 11;5:10199. doi: 10.1038/srep10199. PMID:25960370 doi:http://dx.doi.org/10.1038/srep10199
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