3s4l
From Proteopedia
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==The CRISPR-associated Cas3 HD domain protein MJ0384 from Methanocaldococcus jannaschii== | ==The CRISPR-associated Cas3 HD domain protein MJ0384 from Methanocaldococcus jannaschii== | ||
| - | <StructureSection load='3s4l' size='340' side='right' caption='[[3s4l]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='3s4l' size='340' side='right'caption='[[3s4l]], [[Resolution|resolution]] 2.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3s4l]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3s4l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3m5f 3m5f]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S4L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S4L FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s4l OCA], [https://pdbe.org/3s4l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s4l RCSB], [https://www.ebi.ac.uk/pdbsum/3s4l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s4l ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/CS3HD_METJA CS3HD_METJA] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. Acts as a ssDNA and ssRNA nuclease, with both endo- and 3' to 5' exonuclease activities, acting on substrates with free single-stranded 3' ends. Double-stranded nucleic acids are not substrates. Activity is higher for DNA than RNA. Templates include R-loops (a bubble-like structure formed when ssRNA replaces one strand in a dsDNA, such as crRNA is thought to form with CRISPR target DNA), circular ssDNA, 2',3'-cAMP and 2',3'-cGMP. Probably generates 3'-phosphate and 5'-hydroxyl ends. In the presence of the Cas3 helicase and ATP dsDNA templates are also degraded.<ref>PMID:22009198</ref> |
==See Also== | ==See Also== | ||
| - | *[[Endonuclease|Endonuclease]] | + | *[[Endonuclease 3D structures|Endonuclease 3D structures]] |
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Methanocaldococcus jannaschii DSM 2661]] |
| - | [[Category: | + | [[Category: Brown G]] |
| - | [[Category: | + | [[Category: Edwards A]] |
| - | [[Category: | + | [[Category: Joachimiak A]] |
| - | [[Category: Petit | + | [[Category: Petit P]] |
| - | [[Category: Savchenko | + | [[Category: Savchenko A]] |
| - | [[Category: Yakunin | + | [[Category: Yakunin A]] |
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Current revision
The CRISPR-associated Cas3 HD domain protein MJ0384 from Methanocaldococcus jannaschii
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