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| - | ==CRYSTAL STRUCTURE OF THE ELMO1 PH DOMAIN== | + | ==Crystal Structure of the ELMO1 PH domain== |
| - | <StructureSection load='2vsz' size='340' side='right' caption='[[2vsz]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='2vsz' size='340' side='right'caption='[[2vsz]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2vsz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VSZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VSZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2vsz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VSZ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vsz OCA], [http://pdbe.org/2vsz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vsz RCSB], [http://www.ebi.ac.uk/pdbsum/2vsz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vsz ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vsz OCA], [https://pdbe.org/2vsz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vsz RCSB], [https://www.ebi.ac.uk/pdbsum/2vsz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vsz ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ELMO1_HUMAN ELMO1_HUMAN]] Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.<ref>PMID:11595183</ref> <ref>PMID:12134158</ref> | + | [https://www.uniprot.org/uniprot/ELMO1_HUMAN ELMO1_HUMAN] Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.<ref>PMID:11595183</ref> <ref>PMID:12134158</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vs/2vsz_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vs/2vsz_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Barford, D]] | + | [[Category: Large Structures]] |
| - | [[Category: Cote, J F]] | + | [[Category: Barford D]] |
| - | [[Category: Komander, D]] | + | [[Category: Cote J-F]] |
| - | [[Category: Patel, M]] | + | [[Category: Komander D]] |
| - | [[Category: Apoptosis]]
| + | [[Category: Patel M]] |
| - | [[Category: Dock180]]
| + | |
| - | [[Category: Elmo]]
| + | |
| - | [[Category: Guanine nucleotide exchange factor]]
| + | |
| - | [[Category: Phagocytosis]]
| + | |
| - | [[Category: Phosphoinositide binding]]
| + | |
| - | [[Category: Rac signalling]]
| + | |
| - | [[Category: Sh3-binding]]
| + | |
| Structural highlights
Function
ELMO1_HUMAN Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The mammalian DOCK180 protein belongs to an evolutionarily conserved protein family, which together with ELMO proteins, is essential for activation of Rac GTPase-dependent biological processes. Here, we have analyzed the DOCK180-ELMO1 interaction, and map direct interaction interfaces to the N-terminal 200 amino acids of DOCK180, and to the C-terminal 200 amino acids of ELMO1, comprising the ELMO1 PH domain. Structural and biochemical analysis of this PH domain reveals that it is incapable of phospholipid binding, but instead structurally resembles FERM domains. Moreover, the structure revealed an N-terminal amphiphatic alpha-helix, and point mutants of invariant hydrophobic residues in this helix disrupt ELMO1-DOCK180 complex formation. A secondary interaction between ELMO1 and DOCK180 is conferred by the DOCK180 SH3 domain and proline-rich motifs at the ELMO1 C-terminus. Mutation of both DOCK180-interaction sites on ELMO1 is required to disrupt the DOCK180-ELMO1 complex. Significantly, although this does not affect DOCK180 GEF activity toward Rac in vivo, Rac signaling is impaired, implying additional roles for ELMO in mediating intracellular Rac signaling.
An alpha-helical extension of the ELMO1 pleckstrin homology domain mediates direct interaction to DOCK180 and is critical in Rac signaling.,Komander D, Patel M, Laurin M, Fradet N, Pelletier A, Barford D, Cote JF Mol Biol Cell. 2008 Nov;19(11):4837-51. Epub 2008 Sep 3. PMID:18768751[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gumienny TL, Brugnera E, Tosello-Trampont AC, Kinchen JM, Haney LB, Nishiwaki K, Walk SF, Nemergut ME, Macara IG, Francis R, Schedl T, Qin Y, Van Aelst L, Hengartner MO, Ravichandran KS. CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration. Cell. 2001 Oct 5;107(1):27-41. PMID:11595183
- ↑ Brugnera E, Haney L, Grimsley C, Lu M, Walk SF, Tosello-Trampont AC, Macara IG, Madhani H, Fink GR, Ravichandran KS. Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex. Nat Cell Biol. 2002 Aug;4(8):574-82. PMID:12134158 doi:http://dx.doi.org/10.1038/ncb824
- ↑ Komander D, Patel M, Laurin M, Fradet N, Pelletier A, Barford D, Cote JF. An alpha-helical extension of the ELMO1 pleckstrin homology domain mediates direct interaction to DOCK180 and is critical in Rac signaling. Mol Biol Cell. 2008 Nov;19(11):4837-51. Epub 2008 Sep 3. PMID:18768751 doi:10.1091/mbc.E08-04-0345
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