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1mjn

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[[Image:1mjn.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of the intermediate affinity aL I domain mutant==
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|PDB= 1mjn |SIZE=350|CAPTION= <scene name='initialview01'>1mjn</scene>, resolution 1.30&Aring;
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<StructureSection load='1mjn' size='340' side='right'caption='[[1mjn]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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<table><tr><td colspan='2'>[[1mjn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MJN FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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|GENE= BL21(DE3) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mjn OCA], [https://pdbe.org/1mjn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mjn RCSB], [https://www.ebi.ac.uk/pdbsum/1mjn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mjn ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mjn OCA], [http://www.ebi.ac.uk/pdbsum/1mjn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mjn RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/ITAL_HUMAN ITAL_HUMAN] Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mj/1mjn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mjn ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of the intermediate affinity aL I domain mutant'''
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==See Also==
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*[[Integrin 3D structures|Integrin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The structure of the I domain of integrin alpha L beta 2 bound to the Ig superfamily ligand ICAM-1 reveals the open ligand binding conformation and the first example of an integrin-IgSF interface. The I domain Mg2+ directly coordinates Glu-34 of ICAM-1, and a dramatic swing of I domain residue Glu-241 enables a critical salt bridge. Liganded and unliganded structures for both high- and intermediate-affinity mutant I domains reveal that ligand binding can induce conformational change in the alpha L I domain and that allosteric signals can convert the closed conformation to intermediate or open conformations without ligand binding. Pulling down on the C-terminal alpha 7 helix with introduced disulfide bonds ratchets the beta 6-alpha 7 loop into three different positions in the closed, intermediate, and open conformations, with a progressive increase in affinity.
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Dong YC]]
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1MJN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJN OCA].
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[[Category: Jun CD]]
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[[Category: Liu JH]]
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==Reference==
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[[Category: McCormack A]]
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Structures of the alpha L I domain and its complex with ICAM-1 reveal a shape-shifting pathway for integrin regulation., Shimaoka M, Xiao T, Liu JH, Yang Y, Dong Y, Jun CD, McCormack A, Zhang R, Joachimiak A, Takagi J, Wang JH, Springer TA, Cell. 2003 Jan 10;112(1):99-111. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12526797 12526797]
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[[Category: Shimaoka M]]
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[[Category: Escherichia coli]]
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[[Category: Springer TA]]
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[[Category: Single protein]]
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[[Category: Wang JH]]
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[[Category: Dong, Y C.]]
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[[Category: Xiao T]]
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[[Category: Jun, C D.]]
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[[Category: Yang YT]]
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[[Category: Liu, J H.]]
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[[Category: Zhang RG]]
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[[Category: McCormack, A.]]
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[[Category: Shimaoka, M.]]
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[[Category: Springer, T A.]]
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[[Category: Wang, J H.]]
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[[Category: Xiao, T.]]
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[[Category: Yang, Y T.]]
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[[Category: Zhang, R G.]]
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[[Category: rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:16:56 2008''
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Current revision

Crystal Structure of the intermediate affinity aL I domain mutant

PDB ID 1mjn

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