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| | ==Crystal Structure of Ebola Virus VP40 Dimer== | | ==Crystal Structure of Ebola Virus VP40 Dimer== |
| - | <StructureSection load='4ldb' size='340' side='right' caption='[[4ldb]], [[Resolution|resolution]] 3.10Å' scene=''> | + | <StructureSection load='4ldb' size='340' side='right'caption='[[4ldb]], [[Resolution|resolution]] 3.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ldb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Zebov Zebov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LDB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LDB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ldb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Zaire_ebolavirus Zaire ebolavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LDB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LDB FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ld8|4ld8]], [[4ldd|4ldd]], [[4ldi|4ldi]], [[4ldm|4ldm]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VP40 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186538 ZEBOV])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ldb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ldb OCA], [https://pdbe.org/4ldb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ldb RCSB], [https://www.ebi.ac.uk/pdbsum/4ldb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ldb ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ldb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ldb OCA], [http://pdbe.org/4ldb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ldb RCSB], [http://www.ebi.ac.uk/pdbsum/4ldb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ldb ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/VP40_EBOZM VP40_EBOZM]] Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. The hexamer form seems to be involved in budding. The octamer form binds RNA, and may play a role in genome replication.<ref>PMID:15892969</ref> <ref>PMID:16719918</ref> | + | [https://www.uniprot.org/uniprot/VP40_EBOZM VP40_EBOZM] Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. The hexamer form seems to be involved in budding. The octamer form binds RNA, and may play a role in genome replication.<ref>PMID:15892969</ref> <ref>PMID:16719918</ref> |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Proteins, particularly viral proteins, can be multifunctional, but the mechanisms behind multifunctionality are not fully understood. Here, we illustrate through multiple crystal structures, biochemistry, and cellular microscopy that VP40 rearranges into different structures, each with a distinct function required for the ebolavirus life cycle. A butterfly-shaped VP40 dimer traffics to the cellular membrane. Once there, electrostatic interactions trigger rearrangement of the polypeptide into a linear hexamer. These hexamers construct a multilayered, filamentous matrix structure that is critical for budding and resembles tomograms of authentic virions. A third structure of VP40, formed by a different rearrangement, is not involved in virus assembly but instead uniquely binds RNA to regulate viral transcription inside infected cells. These results provide a functional model for ebolavirus matrix assembly and the other roles of VP40 in the virus life cycle and demonstrate how a single wild-type, unmodified polypeptide can assemble into different structures for different functions. PAPERFLICK:
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| - | Structural Rearrangement of Ebola Virus VP40 Begets Multiple Functions in the Virus Life Cycle.,Bornholdt ZA, Noda T, Abelson DM, Halfmann P, Wood MR, Kawaoka Y, Saphire EO Cell. 2013 Aug 15;154(4):763-74. doi: 10.1016/j.cell.2013.07.015. PMID:23953110<ref>PMID:23953110</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 4ldb" style="background-color:#fffaf0;"></div>
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| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Zebov]] | + | [[Category: Large Structures]] |
| - | [[Category: Ableson, D M]] | + | [[Category: Zaire ebolavirus]] |
| - | [[Category: Bornholdt, Z A]] | + | [[Category: Ableson DM]] |
| - | [[Category: Saphire, E O]] | + | [[Category: Bornholdt ZA]] |
| - | [[Category: Assembly]] | + | [[Category: Saphire EO]] |
| - | [[Category: Viral budding]]
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| - | [[Category: Viral matrix protein]]
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| - | [[Category: Viral protein]]
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| - | [[Category: Viral transcription regulation]]
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