1bfr

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[[Image:1bfr.gif|left|200px]]<br />
 
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<applet load="1bfr" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bfr, resolution 2.94&Aring;" />
 
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'''IRON STORAGE AND ELECTRON TRANSPORT'''<br />
 
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==Overview==
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==IRON STORAGE AND ELECTRON TRANSPORT==
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Crystals of E. coli cytochrome b1, alias bacterioferritin, were grown fr, om a low ionic strength solution. The resulting monoclniic P21 structure, was solved by molecular replacement and refined using noncrystallographi c, symmetries applied to the fundamental unit, consisting of two protein, subunits and a single haem. From the Patterson self-rotation results it, was shown that the asymmetric unit of the monoclinic crystal consists of, 12 such dimers and corresponds to a complete, nearly spherical, molecule, of bacterioferritin (M4 = 450 kDa) of 432 point-group symmetry. It is thus, the most symmetrical cytochrome. As previously determined for the, tetragonal form, the haem is located in a special position on a local, twofold axis of the dimer. A bimetal centre is also observed within the, four-helix bundle of each monomer; a metal-binding site is located on the, fourfold axis.
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<StructureSection load='1bfr' size='340' side='right'caption='[[1bfr]], [[Resolution|resolution]] 2.94&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bfr]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BFR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.94&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bfr OCA], [https://pdbe.org/1bfr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bfr RCSB], [https://www.ebi.ac.uk/pdbsum/1bfr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bfr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BFR_ECOLI BFR_ECOLI] Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex. The mineralized iron core can contain as many as 2700 iron atoms/24-meric molecule.<ref>PMID:10769150</ref> <ref>PMID:14636073</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/1bfr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bfr ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1BFR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MN and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Sites: MA, MB, MC, MD, ME, MF, MG, MH, MI, MJ, MK, ML, MM, MN, MO, MP, MQ, MR, MS, MT, MU, MV, MW and MX. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BFR OCA].
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*[[Ferritin 3D structures|Ferritin 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structure of a monoclinic crystal from of cyctochrome b1 (Bacterioferritin) from E. coli., Dautant A, Meyer JB, Yariv J, Precigoux G, Sweet RM, Kalb AJ, Frolow F, Acta Crystallogr D Biol Crystallogr. 1998 Jan 1;54(Pt 1):16-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9867433 9867433]
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Dautant, A.]]
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[[Category: Dautant A]]
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[[Category: Frolow, F.]]
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[[Category: Frolow F]]
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[[Category: Gilboa, A.J.Kalb.]]
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[[Category: Meyer JB]]
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[[Category: Meyer, J.B.]]
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[[Category: Precigoux G]]
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[[Category: Precigoux, G.]]
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[[Category: Sweet RM]]
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[[Category: Sweet, R.M.]]
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[[Category: Yariv J]]
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[[Category: Yariv, J.]]
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[[Category: HEM]]
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[[Category: MN]]
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[[Category: electron transport]]
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[[Category: iron storage]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:54:24 2007''
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Current revision

IRON STORAGE AND ELECTRON TRANSPORT

PDB ID 1bfr

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