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| | ==GM1 bound form of the ECX AB5 holotoxin== | | ==GM1 bound form of the ECX AB5 holotoxin== |
| - | <StructureSection load='4l6t' size='340' side='right' caption='[[4l6t]], [[Resolution|resolution]] 1.86Å' scene=''> | + | <StructureSection load='4l6t' size='340' side='right'caption='[[4l6t]], [[Resolution|resolution]] 1.86Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4l6t]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L6T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L6T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4l6t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L6T FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.859Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4l63|4l63]], [[2wv6|2wv6]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l6t OCA], [https://pdbe.org/4l6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l6t RCSB], [https://www.ebi.ac.uk/pdbsum/4l6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l6t ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ecxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), ecxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l6t OCA], [http://pdbe.org/4l6t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4l6t RCSB], [http://www.ebi.ac.uk/pdbsum/4l6t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4l6t ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8GAV4_ECOLX Q8GAV4_ECOLX] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| - | [[Category: Beddoe, T]] | + | [[Category: Large Structures]] |
| - | [[Category: Littler, D R]] | + | [[Category: Beddoe T]] |
| - | [[Category: Ng, N M]] | + | [[Category: Littler DR]] |
| - | [[Category: Rossjohn, J]] | + | [[Category: Ng NM]] |
| - | [[Category: Ab5]] | + | [[Category: Rossjohn J]] |
| - | [[Category: Bacterial mmp]]
| + | |
| - | [[Category: Cholera-like toxin]]
| + | |
| - | [[Category: Ganglioside]]
| + | |
| - | [[Category: Gm1]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Matrix metalloproteinase]]
| + | |
| - | [[Category: Metzincin]]
| + | |
| - | [[Category: Ob fold]]
| + | |
| - | [[Category: Oligosaccharide complex]]
| + | |
| - | [[Category: Pentasacharide]]
| + | |
| - | [[Category: Protease]]
| + | |
| - | [[Category: Toxilysin]]
| + | |
| - | [[Category: Toxin]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
4l6t is a 6 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 1.859Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
Q8GAV4_ECOLX
Publication Abstract from PubMed
AB5 toxins are composed of an enzymatic A subunit that disrupts cellular function associated with a pentameric B subunit required for host cell invasion. EcxAB is an AB5 toxin isolated from clinical strains of Escherichia coli classified as part of the cholera family due to B subunit homology. Cholera-group toxins have catalytic ADP-ribosyltransferases as their A subunits, so it was surprising that EcxA did not. We confirmed that EcxAB self-associates as a functional toxin and obtained its structure. EcxAB is a prototypical member of a hybrid AB5 toxin family containing metzincin-type metalloproteases as their active A subunit paired to a cholera-like B subunit. Furthermore, EcxA is distinct from previously characterized proteases and thus founds an AB5-associated metzincin family that we term the toxilysins. EcxAB provides the first observation of conserved B subunit usage across different AB5 toxin families and provides evidence that the intersubunit interface of these toxins is far more permissive than previously supposed.
EcxAB Is a Founding Member of a New Family of Metalloprotease AB Toxins with a Hybrid Cholera-like B Subunit.,Ng NM, Littler DR, Paton AW, Le Nours J, Rossjohn J, Paton JC, Beddoe T Structure. 2013 Oct 1. pii: S0969-2126(13)00345-6. doi:, 10.1016/j.str.2013.08.024. PMID:24095060[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ng NM, Littler DR, Paton AW, Le Nours J, Rossjohn J, Paton JC, Beddoe T. EcxAB Is a Founding Member of a New Family of Metalloprotease AB Toxins with a Hybrid Cholera-like B Subunit. Structure. 2013 Oct 1. pii: S0969-2126(13)00345-6. doi:, 10.1016/j.str.2013.08.024. PMID:24095060 doi:http://dx.doi.org/10.1016/j.str.2013.08.024
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