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| | ==Crystal Structure of Helicobacter pylori Urease Accessory Protein UreF/H/G complex== | | ==Crystal Structure of Helicobacter pylori Urease Accessory Protein UreF/H/G complex== |
| - | <StructureSection load='4hi0' size='340' side='right' caption='[[4hi0]], [[Resolution|resolution]] 2.35Å' scene=''> | + | <StructureSection load='4hi0' size='340' side='right'caption='[[4hi0]], [[Resolution|resolution]] 2.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4hi0]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_pylori Campylobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HI0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HI0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4hi0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HI0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HI0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sf5|3sf5]], [[3o1q|3o1q]], [[2wgl|2wgl]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HP_0069, ureF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Campylobacter pylori]), HP_0067, ureH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Campylobacter pylori]), HP_0068, ureG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Campylobacter pylori])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hi0 OCA], [https://pdbe.org/4hi0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hi0 RCSB], [https://www.ebi.ac.uk/pdbsum/4hi0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hi0 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hi0 OCA], [http://pdbe.org/4hi0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hi0 RCSB], [http://www.ebi.ac.uk/pdbsum/4hi0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hi0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/UREF_HELPY UREF_HELPY]] Required for maturation of urease via the functional incorporation of the urease nickel metallocenter (By similarity).[HAMAP-Rule:MF_01385] [[http://www.uniprot.org/uniprot/UREG_HELPY UREG_HELPY]] Facilitates the functional incorporation of the urease nickel metallocenter. This process requires GTP hydrolysis, probably effectuated by UreG (By similarity).[HAMAP-Rule:MF_01389] [[http://www.uniprot.org/uniprot/UREH_HELPY UREH_HELPY]] Required for maturation of urease via the functional incorporation of the urease nickel metallocenter (By similarity).[HAMAP-Rule:MF_01384] | + | [https://www.uniprot.org/uniprot/UREF_HELPY UREF_HELPY] Required for maturation of urease via the functional incorporation of the urease nickel metallocenter (By similarity).[HAMAP-Rule:MF_01385] |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Urease is a metalloenzyme essential for the survival of Helicobacter pylori in acidic gastric environment. Maturation of urease involves carbamylation of Lys219 and insertion of two nickel ions at its active site. This process requires GTP hydrolysis and the formation of a preactivation complex consisting of apo-urease and urease accessory proteins UreF, UreH, and UreG. UreF and UreH form a complex to recruit UreG, which is a SIMIBI class GTPase, to the preactivation complex. We report here the crystal structure of the UreG/UreF/UreH complex, which illustrates how UreF and UreH facilitate dimerization of UreG, and assembles its metal binding site by juxtaposing two invariant Cys66-Pro67-His68 metal binding motif at the interface to form the (UreG/UreF/UreH)2 complex. Interaction studies revealed that addition of nickel and GTP to the UreG/UreF/UreH complex releases a UreG dimer that binds a nickel ion at the dimeric interface. Substitution of Cys66 and His68 with alanine abolishes the formation of the nickel-charged UreG dimer. This nickel-charged UreG dimer can activate urease in vitro in the presence of the UreF/UreH complex. Static light scattering and atomic absorption spectroscopy measurements demonstrated that the nickel-charged UreG dimer, upon GTP hydrolysis, reverts to its monomeric form and releases nickel to urease. Based on our results, we propose a mechanism on how urease accessory proteins facilitate maturation of urease.
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| - | Structure of UreG/UreF/UreH complex reveals how urease accessory proteins facilitate maturation of Helicobacter pylori urease.,Fong YH, Wong HC, Yuen MH, Lau PH, Chen YW, Wong KB PLoS Biol. 2013 Oct;11(10):e1001678. doi: 10.1371/journal.pbio.1001678. Epub 2013, Oct 8. PMID:24115911<ref>PMID:24115911</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 4hi0" style="background-color:#fffaf0;"></div>
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| | ==See Also== | | ==See Also== |
| | *[[Urease accessory protein|Urease accessory protein]] | | *[[Urease accessory protein|Urease accessory protein]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Campylobacter pylori]] | + | [[Category: Helicobacter pylori 26695]] |
| - | [[Category: Chen, Y W]] | + | [[Category: Large Structures]] |
| - | [[Category: Fong, Y H]] | + | [[Category: Chen YW]] |
| - | [[Category: Wong, K B]] | + | [[Category: Fong YH]] |
| - | [[Category: Cytosol]] | + | [[Category: Wong KB]] |
| - | [[Category: Metal binding protein]]
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| - | [[Category: Metallochaperone]]
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| - | [[Category: Urease]]
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