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| - | [[Image:1mn0.jpg|left|200px]] | |
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| - | {{Structure
| + | ==Crystal structure of galactose mutarotase from lactococcus lactis complexed with D-xylose== |
| - | |PDB= 1mn0 |SIZE=350|CAPTION= <scene name='initialview01'>1mn0</scene>, resolution 1.90Å
| + | <StructureSection load='1mn0' size='340' side='right'caption='[[1mn0]], [[Resolution|resolution]] 1.90Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene>
| + | <table><tr><td colspan='2'>[[1mn0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MN0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MN0 FirstGlance]. <br> |
| - | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Aldose_1-epimerase Aldose 1-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.3 5.1.3.3] </span>
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | |GENE= GALM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 Lactococcus lactis])
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mn0 OCA], [https://pdbe.org/1mn0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mn0 RCSB], [https://www.ebi.ac.uk/pdbsum/1mn0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mn0 ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=[[1mmu|1MMU]], [[1mmx|1MMX]], [[1mmy|1MMY]], [[1mmz|1MMZ]]
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mn0 OCA], [http://www.ebi.ac.uk/pdbsum/1mn0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mn0 RCSB]</span>
| + | == Function == |
| - | }}
| + | [https://www.uniprot.org/uniprot/Q9ZB17_9LACT Q9ZB17_9LACT] |
| | + | == Evolutionary Conservation == |
| | + | [[Image:Consurf_key_small.gif|200px|right]] |
| | + | Check<jmol> |
| | + | <jmolCheckbox> |
| | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mn/1mn0_consurf.spt"</scriptWhenChecked> |
| | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | + | <text>to colour the structure by Evolutionary Conservation</text> |
| | + | </jmolCheckbox> |
| | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mn0 ConSurf]. |
| | + | <div style="clear:both"></div> |
| | | | |
| - | '''Crystal structure of galactose mutarotase from lactococcus lactis complexed with D-xylose'''
| + | ==See Also== |
| - | | + | *[[Galactose mutarotase|Galactose mutarotase]] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | Galactose mutarotase catalyzes the conversion of beta-D-galactose to alpha-D-galactose in the Leloir pathway for galactose metabolism. The high resolution x-ray structure of the dimeric enzyme from Lactococcus lactis was recently solved and shown to be topologically similar to the 18-stranded, anti-parallel beta-motif observed for domain 5 of beta-galactosidase. In addition to determining the overall molecular fold of galactose mutarotase, this initial investigation also provided a detailed description of the electrostatic interactions between the enzyme and its physiologically relevant substrate, galactose. Specifically, the side chains of His-96 and His-170 were shown to be located within hydrogen bonding distance to the C-5 oxygen of the substrate, while the carboxylate of Glu-304 was positioned near the C-1 hydroxyl group of the sugar. On the basis of this initial study, a possible role for Glu-304 as the general acid/base group in catalysis was put forth. Here we describe the combined x-ray crystallographic and kinetic analyses of L. lactis galactose mutarotase complexed with D-glucose, D-fucose, D-quinovose, L-arabinose, or D-xylose. These investigations have revealed that there are several distinct binding modes for these sugars, which are dependent upon the spatial orientation of the C-4 hydroxyl group. In those sugars with the same C-4 hydroxyl group orientation as galactose, their C-1 hydroxyl groups are invariably located near Glu-304. For those sugars, which have the same C-4 hydroxyl group configuration as glucose, the C-1 hydroxyls are typically located near Asp-243. These different binding modes correlate with both the observed kinetic parameters and the presence or absence of a hydrogen bond between the guanidinium group of Arg-71 and the C-4 hydroxyl group of the sugar ligand. | + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1MN0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MN0 OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | Structural and kinetic studies of sugar binding to galactose mutarotase from Lactococcus lactis., Thoden JB, Kim J, Raushel FM, Holden HM, J Biol Chem. 2002 Nov 22;277(47):45458-65. Epub 2002 Sep 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12218067 12218067]
| + | |
| - | [[Category: Aldose 1-epimerase]]
| + | |
| | [[Category: Lactococcus lactis]] | | [[Category: Lactococcus lactis]] |
| - | [[Category: Single protein]] | + | [[Category: Large Structures]] |
| - | [[Category: Holden, H M.]] | + | [[Category: Holden HM]] |
| - | [[Category: Kim, J.]] | + | [[Category: Kim J]] |
| - | [[Category: Raushel, F M.]] | + | [[Category: Raushel FM]] |
| - | [[Category: Thoden, J B.]] | + | [[Category: Thoden JB]] |
| - | [[Category: epimerase]]
| + | |
| - | [[Category: galactosemia]]
| + | |
| - | [[Category: sugar binding]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:18:19 2008''
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