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3wxj

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Crystal structure of trypanosoma brucei gambiense glycerol kinase in complex with glycerol 3-phosphate

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 ==Crystal structure of trypanosoma brucei gambiense glycerol kinase in complex with glycerol 3-phosphate==
-<StructureSection load='3wxj' size='340' side='right' caption='[[3wxj]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+<StructureSection load='3wxj' size='340' side='right'caption='[[3wxj]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3wxj]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WXJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3wxj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_gambiense Trypanosoma brucei gambiense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WXJ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G3P:SN-GLYCEROL-3-PHOSPHATE'>G3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wxi|3wxi]], [[3wxk|3wxk]], [[3wxl|3wxl]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G3P:SN-GLYCEROL-3-PHOSPHATE'>G3P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycerol_kinase Glycerol kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.30 2.7.1.30] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wxj OCA], [https://pdbe.org/3wxj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wxj RCSB], [https://www.ebi.ac.uk/pdbsum/3wxj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wxj ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wxj OCA], [http://pdbe.org/3wxj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wxj RCSB], [http://www.ebi.ac.uk/pdbsum/3wxj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wxj ProSAT]</span></td></tr>
 </table>
-<div style="background-color:#fffaf0;">
+== Function ==
-== Publication Abstract from PubMed ==
+[https://www.uniprot.org/uniprot/D3KVM3_TRYBG D3KVM3_TRYBG]
-The glycerol kinase (GK) of African human trypanosomes is compartmentalized in their glycosomes. Unlike the host GK, which under physiological conditions catalyzes only the forward reaction (ATP-dependent glycerol phosphorylation), trypanosome GK can additionally catalyze the reverse reaction. In fact, owing to this unique reverse catalysis, GK is potentially essential for the parasites survival in the human host, hence a promising drug target. The mechanism of its reverse catalysis was unknown; therefore, it was not clear if this ability was purely due to its localization in the organelles or whether structure-based catalytic differences also contribute. To investigate this lack of information, the X-ray crystal structure of this protein was determined up to 1.90 A resolution, in its unligated form and in complex with three natural ligands. These data, in conjunction with results from structure-guided mutagenesis suggests that the trypanosome GK is possibly a transiently autophosphorylating threonine kinase, with the catalytic site formed by non-conserved residues. Our results provide a series of structural peculiarities of this enzyme, and gives unexpected insight into the reverse catalysis mechanism. Together, they provide an encouraging molecular framework for the development of trypanosome GK-specific inhibitors, which may lead to the design of new and safer trypanocidal drug(s).
-Molecular basis for the reverse reaction of African human trypanosomes glycerol kinase.,Balogun EO, Inaoka DK, Shiba T, Kido Y, Tsuge C, Nara T, Aoki T, Honma T, Tanaka A, Inoue M, Matsuoka S, Michels PA, Kita K, Harada S Mol Microbiol. 2014 Dec;94(6):1315-29. doi: 10.1111/mmi.12831. Epub 2014 Nov 4. PMID:25315291<ref>PMID:25315291</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 3wxj" style="background-color:#fffaf0;"></div>
 ==See Also==
 *[[Glycerol kinase|Glycerol kinase]]
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Glycerol kinase]]
+[[Category: Large Structures]]
-[[Category: Aoki, T]]
+[[Category: Trypanosoma brucei gambiense]]
-[[Category: Balogun, E O]]
+[[Category: Aoki T]]
-[[Category: Harada, S]]
+[[Category: Balogun EO]]
-[[Category: Honma, T]]
+[[Category: Harada S]]
-[[Category: Inaoka, D K]]
+[[Category: Honma T]]
-[[Category: Inoue, M]]
+[[Category: Inaoka DK]]
-[[Category: Kido, Y]]
+[[Category: Inoue M]]
-[[Category: Kita, K]]
+[[Category: Kido Y]]
-[[Category: Matsuoka, S]]
+[[Category: Kita K]]
-[[Category: Michels, P A.M]]
+[[Category: Matsuoka S]]
-[[Category: Nara, T]]
+[[Category: Michels PAM]]
-[[Category: Shiba, T]]
+[[Category: Nara T]]
-[[Category: Tanaka, A]]
+[[Category: Shiba T]]
-[[Category: Tsuge, C]]
+[[Category: Tanaka A]]
-[[Category: Glycosome]]
+[[Category: Tsuge C]]
-[[Category: Sugar kinase superfamily]]
-[[Category: Transferase]]
-[[Category: Trypanosoma]]

3wxj

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Crystal structure of trypanosoma brucei gambiense glycerol kinase in complex with glycerol 3-phosphate

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