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| | ==Crystallographic structure of the pseudo-Signaling State of the BLUF Photoreceptor PixD (slr1694) Y8F mutant== | | ==Crystallographic structure of the pseudo-Signaling State of the BLUF Photoreceptor PixD (slr1694) Y8F mutant== |
| - | <StructureSection load='3mzi' size='340' side='right' caption='[[3mzi]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='3mzi' size='340' side='right'caption='[[3mzi]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3mzi]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Aphanocapsa_sp._(strain_n-1) Aphanocapsa sp. (strain n-1)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MZI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MZI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3mzi]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MZI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MZI FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.304Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2hfn|2hfn]], [[2hfo|2hfo]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">slr1694 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1148 Aphanocapsa sp. (strain N-1)])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mzi OCA], [https://pdbe.org/3mzi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mzi RCSB], [https://www.ebi.ac.uk/pdbsum/3mzi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mzi ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mzi OCA], [http://pdbe.org/3mzi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3mzi RCSB], [http://www.ebi.ac.uk/pdbsum/3mzi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3mzi ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/P74295_SYNY3 P74295_SYNY3] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bauer, C E]] | + | [[Category: Large Structures]] |
| - | [[Category: Yuan, H]] | + | [[Category: Synechocystis sp. PCC 6803]] |
| - | [[Category: Phototaxis]] | + | [[Category: Bauer CE]] |
| - | [[Category: Protein binding]] | + | [[Category: Yuan H]] |
| - | [[Category: Signaling state]]
| + | |
| Structural highlights
Function
P74295_SYNY3
Publication Abstract from PubMed
PixD (Slr1694) is a BLUF (blue-light-using FAD) photoreceptor used by the cyanobacterium Synechocystis sp. PCC6803 to control phototaxis toward blue light. In this study, we probe the involvement of a conserved Tyr8-Gln50-Met93 triad in promoting an output signal upon blue light excitation of the bound flavin. Analysis of acrylamide quenching of Trp91 fluorescence shows that the side chain of this residue remains partially solvent exposed in both the lit and dark states. Mutational analysis demonstrates that substitution mutations at Tyr8 and Gln50 result in the loss of the photocycle while a mutation of Met93 does not appreciably disturb the formation of the light-excited state and only minimally accelerates its decay from 5.7 to 4.5 s. However, mutations of Tyr8, Gln50, and Met93 disrupt the ability of PixD dimers to interact with PixE to form a higher-order PixD(10)-PixE(5) complex, which is indicative of a lit conformational state. Solution nuclear magnetic resonance spectroscopy and X-ray crystallographic analyses confirm that a Tyr8 to Phe mutation is locked in a pseudo-light-excited state revealing flexible areas in PixD that likely constitute part of an output signal upon light excitation of wild-type PixD.
Mutational and Structural Studies of the PixD BLUF Output Signal That Affects Light-Regulated Interactions with PixE.,Yuan H, Dragnea V, Wu Q, Gardner KH, Bauer CE Biochemistry. 2011 Jul 26;50(29):6365-75. Epub 2011 Jun 28. PMID:21688827[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yuan H, Dragnea V, Wu Q, Gardner KH, Bauer CE. Mutational and Structural Studies of the PixD BLUF Output Signal That Affects Light-Regulated Interactions with PixE. Biochemistry. 2011 Jul 26;50(29):6365-75. Epub 2011 Jun 28. PMID:21688827 doi:10.1021/bi200701d
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