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| | ==Crystal structure analysis of the plant protease Deg1== | | ==Crystal structure analysis of the plant protease Deg1== |
| - | <StructureSection load='3qo6' size='340' side='right' caption='[[3qo6]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='3qo6' size='340' side='right'caption='[[3qo6]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3qo6]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath] and [http://en.wikipedia.org/wiki/Miscellaneous_nucleic_acid Miscellaneous nucleic acid]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QO6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3qo6]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QO6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QO6 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">At3g27925, Deg1, DEGP, DEGP1, K16N12.18 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qo6 OCA], [https://pdbe.org/3qo6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qo6 RCSB], [https://www.ebi.ac.uk/pdbsum/3qo6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qo6 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qo6 OCA], [http://pdbe.org/3qo6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qo6 RCSB], [http://www.ebi.ac.uk/pdbsum/3qo6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qo6 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/DEGP1_ARATH DEGP1_ARATH]] Serine protease that is required at high temperature. May be involved in the degradation of damaged proteins. In vivo, can degrade beta-casein. | + | [https://www.uniprot.org/uniprot/DEGP1_ARATH DEGP1_ARATH] Serine protease that is required at high temperature. May be involved in the degradation of damaged proteins. In vivo, can degrade beta-casein. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| - | [[Category: Miscellaneous nucleic acid]] | + | [[Category: Large Structures]] |
| - | [[Category: Clausen, T]] | + | [[Category: Unidentified]] |
| - | [[Category: Htra]] | + | [[Category: Clausen T]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Ph-sensor]]
| + | |
| - | [[Category: Photosynthesis]]
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| - | [[Category: Protease]]
| + | |
| Structural highlights
Function
DEGP1_ARATH Serine protease that is required at high temperature. May be involved in the degradation of damaged proteins. In vivo, can degrade beta-casein.
Publication Abstract from PubMed
Deg1 is a chloroplastic protease involved in maintaining the photosynthetic machinery. Structural and biochemical analyses reveal that the inactive Deg1 monomer is transformed into the proteolytically active hexamer at acidic pH. The change in pH is sensed by His244, which upon protonation, repositions a specific helix to trigger oligomerization. This system ensures selective activation of Deg1 during daylight, when acidification of the thylakoid lumen occurs and photosynthetic proteins are damaged.
Structural adaptation of the plant protease Deg1 to repair photosystem II during light exposure.,Kley J, Schmidt B, Boyanov B, Stolt-Bergner PC, Kirk R, Ehrmann M, Knopf RR, Naveh L, Adam Z, Clausen T Nat Struct Mol Biol. 2011 Jun;18(6):728-31. Epub 2011 May 1. PMID:21532594[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kley J, Schmidt B, Boyanov B, Stolt-Bergner PC, Kirk R, Ehrmann M, Knopf RR, Naveh L, Adam Z, Clausen T. Structural adaptation of the plant protease Deg1 to repair photosystem II during light exposure. Nat Struct Mol Biol. 2011 Jun;18(6):728-31. Epub 2011 May 1. PMID:21532594 doi:10.1038/nsmb.2055
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