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| | ==Crystal structure of the centromere-binding protein ParB from plasmid pCXC100== | | ==Crystal structure of the centromere-binding protein ParB from plasmid pCXC100== |
| - | <StructureSection load='3no7' size='340' side='right' caption='[[3no7]], [[Resolution|resolution]] 1.40Å' scene=''> | + | <StructureSection load='3no7' size='340' side='right'caption='[[3no7]], [[Resolution|resolution]] 1.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3no7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33973 Atcc 33973]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NO7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3no7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leifsonia_xyli_subsp._cynodontis Leifsonia xyli subsp. cynodontis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NO7 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pCXC100 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=31966 ATCC 33973])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3no7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3no7 OCA], [http://pdbe.org/3no7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3no7 RCSB], [http://www.ebi.ac.uk/pdbsum/3no7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3no7 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3no7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3no7 OCA], [https://pdbe.org/3no7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3no7 RCSB], [https://www.ebi.ac.uk/pdbsum/3no7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3no7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q6EEF9_LEIXC Q6EEF9_LEIXC] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 33973]] | + | [[Category: Large Structures]] |
| - | [[Category: Ye, K]] | + | [[Category: Leifsonia xyli subsp. cynodontis]] |
| - | [[Category: Dna binding protein]] | + | [[Category: Ye K]] |
| - | [[Category: Ribbon-helix-helix]]
| + | |
| Structural highlights
Function
Q6EEF9_LEIXC
Publication Abstract from PubMed
Plasmid pCXC100 from the Gram-positive bacterium Leifsonia xyli subsp. cynodontis uses a type Ib partition system that includes a centromere region, a Walker-type ATPase ParA and a centromere-binding protein ParB for stable segregation. However, ParB shows no detectable sequence homology to any DNA-binding motif. Here, we study the ParB centromere interaction by structural and biochemical approaches. The crystal structure of the C-terminal DNA-binding domain of ParB at 1.4 A resolution reveals a dimeric ribbon-helix-helix (RHH) motif, supporting the prevalence of RHH motif in centromere binding. Using hydroxyl radical footprinting and quantitative binding assays, we show that the centromere core comprises nine uninterrupted 9-nt direct repeats that can be successively bound by ParB dimers in a cooperative manner. However, the interaction of ParB with a single subsite requires 18 base pairs covering one immediate repeat as well as two halves of flanking repeats. Through mutagenesis, sequence specificity was determined for each position of an 18-bp subsite. These data suggest an unique centromere recognition mechanism by which the repeat sequence is jointly specified by adjacent ParB dimers bound to an overlapped region.
Crystal structure and centromere binding of the plasmid segregation protein ParB from pCXC100.,Huang L, Yin P, Zhu X, Zhang Y, Ye K Nucleic Acids Res. 2010 Dec 1. PMID:21123191[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Huang L, Yin P, Zhu X, Zhang Y, Ye K. Crystal structure and centromere binding of the plasmid segregation protein ParB from pCXC100. Nucleic Acids Res. 2010 Dec 1. PMID:21123191 doi:10.1093/nar/gkq915
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